Oligomerization and activation of caspase-9, induced by Apaf-1 CARD.

  title={Oligomerization and activation of caspase-9, induced by Apaf-1 CARD.},
  author={Eric N Shiozaki and Jijie Chai and Yigong Shi},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={99 7},
Apaf-1 facilitates the proteolytic activation of procaspase-9 and maintains the hyperactive state of the processed caspase-9. The underlying molecular mechanisms for these activities remain poorly characterized. Here we report that the isolated Apaf-1 caspase recruitment domain (CARD) forms a large hetero-oligomer with the active caspase-9. The catalytic activity of caspase-9 is significantly enhanced in this complex, demonstrating that Apaf-1 CARD allosterically up-regulates caspase-9 activity… CONTINUE READING