The structure of the Rous sarcoma virus envelope glycoprotein complex was studied by sedimentation gradient centrifugation analyses of detergent-solubilized wild-type and mutant envelope (env) gene products. These studies show that the envelope glycoprotein forms an oligomer during biosynthesis, which is most likely a trimer, and that this is the form of… (More)
FIG. 4. Analysis of the oligomeric nature of transport-defective RSV env mutants. Lysates from CV-1 cells expressing the C3 (A and B) or X1 (C and D) mutant RSV envelope proteins from SV40 vectors were analyzed as in Fig. 1. Cells were lysed immediately after a 5-min pulse (A and C) or following a 1-hr chase at 37°C (B and D).