Oligodeoxynucleotide-modified capillary for electrophoretic separation of single-stranded DNAs with a single-base difference.

@article{Anada2003OligodeoxynucleotidemodifiedCF,
  title={Oligodeoxynucleotide-modified capillary for electrophoretic separation of single-stranded DNAs with a single-base difference.},
  author={Takahisa Anada and Masako Ogawa and Hisashi Yokomizo and Yoshihisa Ozaki and Tohru Takarada and Yoshiki Katayama and Mizuo Maeda},
  journal={Analytical sciences : the international journal of the Japan Society for Analytical Chemistry},
  year={2003},
  volume={19 1},
  pages={73-7}
}
We describe here a method of affinity capillary electrophoresis in which oligodeoxynucleotide (ODN) was immobilized onto the inner surface of the capillary. The immobilized ODN functioned successfully as an affinity ligand for sequence-based DNA separation. Six- or 12-mer ODN with a sequence complementary to one of the c-K-ras gene was used as an immobilized ligand. When the 12-mer ODN was used, the detection peak for the complementary ODN disappeared selectively, while the single-base mutant… CONTINUE READING
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