Oligodeoxynucleotide-modified capillary for electrophoretic separation of single-stranded DNAs with a single-base difference.

  title={Oligodeoxynucleotide-modified capillary for electrophoretic separation of single-stranded DNAs with a single-base difference.},
  author={Takahisa Anada and Masako Ogawa and Hisashi Yokomizo and Yoshihisa Ozaki and Tohru Takarada and Yoshiki Katayama and Mizuo Maeda},
  journal={Analytical sciences : the international journal of the Japan Society for Analytical Chemistry},
  volume={19 1},
We describe here a method of affinity capillary electrophoresis in which oligodeoxynucleotide (ODN) was immobilized onto the inner surface of the capillary. The immobilized ODN functioned successfully as an affinity ligand for sequence-based DNA separation. Six- or 12-mer ODN with a sequence complementary to one of the c-K-ras gene was used as an immobilized ligand. When the 12-mer ODN was used, the detection peak for the complementary ODN disappeared selectively, while the single-base mutant… CONTINUE READING
1 Citations
12 References
Similar Papers


Publications citing this paper.


Publications referenced by this paper.
Showing 1-10 of 12 references

Biochemistry, 1974, 13, 2579

  • H. Krakauer
  • 2003


  • T. Anada, Y. Ozaki, T. Takarada, Y. Katayama, M. Maeda
  • Sci.,
  • 2001


  • Y. Katayama, T. Arisawa, Y. Ozaki, M. Maeda
  • Lett.,
  • 2000


  • A. Z. Li, H. Huang, X. Re, L. J. Qi, K. A. Marx
  • J.,
  • 1998

and P

  • U. Landegren, M. Nilsson
  • Y. Kwok, Genome Res.,
  • 1998


  • A. T. Wooley, R. A. Mathies
  • Natl. Acad. Sci. USA,
  • 1994

and A

  • D. J. Harrison, K. Fluri, K. Seiler, Z. Fan, C. S. Effenhauser
  • Manz, Science,
  • 1993

and R

  • H. Land, L. F. Parada
  • A. Weinberg, Science,
  • 1983

and C

  • H. Nenne
  • H. Zimmer, Biopolymers,
  • 1966

and K

  • N. Risc
  • Merikangas, Science, 1996, 273,
  • 1516

Similar Papers

Loading similar papers…