Oligodendroglial development in human fetal cerebrum.

@article{Rivkin1995OligodendroglialDI,
  title={Oligodendroglial development in human fetal cerebrum.},
  author={Michael J. Rivkin and Judy Flax and Robin L Mozell and Rapin Osathanondh and Joseph J. Volpe and L. Villa-Komaroff},
  journal={Annals of neurology},
  year={1995},
  volume={38 1},
  pages={92-101}
}
We have successfully established mixed glial cell primary cultures prepared from individual fetal human brains (15-18 weeks' gestation in age). Cultures were maintained for as long as 3 months in either 10% fetal calf serum (FCS) or serum-free chemically defined medium (CDM). By morphological and immunohistochemical criteria, the precursor cell for human oligodendrocytes (O-2A cell) was identified. This cell exhibited the bipolar morphology and A2B5-positive (A2B5+) immunoreactivity typical of… CONTINUE READING
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