Olfactory receptor neurons exist as distinct subclasses of immature and mature cells in primary culture

@article{Cunningham1999OlfactoryRN,
  title={Olfactory receptor neurons exist as distinct subclasses of immature and mature cells in primary culture},
  author={Anne M. Cunningham and Paul B. Manis and R. R. Reed and G. V. Ronnett},
  journal={Neuroscience},
  year={1999},
  volume={93},
  pages={1301-1312}
}
Characteristics of odorant elicited calcium changes in cultured human olfactory neurons
TLDR
C cultured cells established from olfactory biopsies retain some of the in vivo odorant response characteristics of acutely isolated cells from the adult Olfactory epithelium, which has important ramifications for investigation of olfaction function and dysfunction using biopsy procedures and in vitro assays of odor sensitivity.
A long-term culture system for olfactory explants with intrinsically fluorescent cell populations.
TLDR
The results demonstrate that GFP-labeled olfactory sensory neurons differentiate in the established organotypic cultures, which thus appear to be a useful tool to monitor and to manipulate the processes underlying the axonal wiring between the Olfactory epithelium and bulb.
Primary culture of embryonic rat olfactory receptor neurons
TLDR
A logistic regression analysis indicated a significant difference between both embryonic d in the response to FSK + IBMX, and two primary rat olfactory explant cultures of different embryonic d were established, comprising epithelium and bulb.
Primary Cultures of Olfactory Neurons from the Avian Olfactory Epithelium.
  • G. Gomez
  • Biology
    Methods in molecular biology
  • 2018
TLDR
This chapter describes a technique for culture of olfactory neurons from the avian Olfactory epithelium of embryonic chickens that enables the controlled laboratory study of a critical sensory system that is unstudied in this major vertebrate class.
Promotion of Olfactory Receptor Neuron Differentiation of Olfactory Neuroepithelial Cells by Using Chitosan Solution
TLDR
Experimental results revealed that soluble chitosan promoted differentiation of olfactory neuroepithelial cells based on its role in ofactory receptor neuron differentiation, neurite outgrowth, and signal transduction apparatus expressions.
Maintaining epitheliopoietic potency when culturing olfactory progenitors
Odorant receptor expression as a function of neuronal maturity in the adult rodent olfactory system
TLDR
It is determined that onset of P2 OR expression is slightly delayed when evaluated in the context of neuronal differentiation, and the expression of OR+ OSNs in the OE of rats via in situ hybridization with a panel of eight ORs followed by OMP immunostaining is defined.
Atrial Natriuretic Peptide Type C Induces a Cell-Cycle Switch from Proliferation to Differentiation in Brain-Derived Neurotrophic Factor- or Nerve Growth Factor-Primed Olfactory Receptor Neurons
TLDR
CNP may function in the postnatal brain to regulate the exit from the cell cycle in neuronal precursor cells, and is accompanied by changes in cell-cycle proteins that suggest possible mechanisms for these effects.
Establishment of primary cultures of rat olfactory bulb under serum-free conditions for studies of cellular injury
TLDR
The serum-free culture developed here is amenable to morphological and high throughput neurochemical analyses of mechanisms contributing to the injury of OB neurones in vitro and revealed the presence of TUNEL-positive cells and increases in intracellular free calcium, both of which were antagonised by CNQX.
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References

SHOWING 1-10 OF 53 REFERENCES
Factors affecting neuronal birth and death in the mammalian olfactory epithelium.
TLDR
In vitro studies have confirmed that neuronal cells of the olfactory epithelium undergo apoptotic death and have permitted identification of several polypeptide growth factors that promote survival of a fraction of ORNs.
Regulation of neurogenesis and neuronal differentiation in primary and immortalized cells from mouse olfactory epithelium.
TLDR
To determine whether the basal cell-to-INP transition, or proliferation and neuronal differentiation of the INP, are regulated by crucial growth factors or cellular interactions, various polypeptide growth factors and extracellular matrix proteins are testing their effects on OE neurogenesis in vitro.
Neurogenesis and neuron regeneration in the olfactory system of mammals. I. Morphological aspects of differentiation and structural organization of the olfactory sensory neurons
TLDR
The morphological stages of maturation and ageing of this exceptional neuron have been described both at light and electron microscopical levels and the neural elements have been classified as: basal cells proper, globose basal cells, and neurons.
Purified cultures of keratin‐positive olfactory epithelial cells: Identification of a subset as neuronal supporting (Sustentacular) cells
  • S. Pixley
  • Biology
    Journal of neuroscience research
  • 1992
TLDR
This culture system gives novel insights into olfactory epithelial cell physiology, and allows culture of these cells for further studies examining regulation of differentiation, and supports the proposal that the majority of cells were the dark Olfactory basal cells.
The expression of the growth associated protein B50/GAP43 in the olfactory system of neonatal and adult rats
TLDR
Comparison of the expression of B50/GAP43 and the olfactory marker protein (OMP), a polypeptide only present in mature OMP-positive neurons, revealed that during postnatal development of the Olfactory system these 2 proteins are expressed in a nearly reciprocal fashion.
The Dynamics of Cell Death in the Olfactory Epithelium
TLDR
A striking wave of enhanced cell death of 6- to 7-day-old neurons over the contralateral levels occurred ipsilaterally in both the 12-day and 7-week postbulbectomy animals.
Sequential expression of Trks A, B, and C in the regenerating olfactory neuroepithelium
TLDR
This is the first population of neurons of a single lineage in which Trks A, B, and C and the INGFR have been demonstrated to be expressed sequentially during neuronal division, commitment, and differentiation and to be fully capable of transducing cellular signals causing phenotypic changes in differentiation state.
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