Olfaction: Mosquito receptor for human-sweat odorant

  title={Olfaction: Mosquito receptor for human-sweat odorant},
  author={Elissa A. Hallem and A Nicole Fox and Laurence J. Zwiebel and John R. Carlson},
Female Anopheles mosquitoes, the world's most important vector of Plasmodium falciparum malaria, locate their human hosts primarily through olfactory cues, but the molecular mechanisms that underlie this recognition are a mystery. Here we show that the Anopheles gambiae protein AgOr1, a female-specific member of a family of putative odorant receptors, responds to a component of human sweat. Compounds designed to activate or block receptors of this type could function as attractants for trapping… Expand
Odourant reception in the malaria mosquito Anopheles gambiae
This analysis of the Anopheles gambiae receptors permits a comparison with the corresponding Drosophila melanogaster odorant receptor repertoire and finds that odorants are differentially encoded by the two species in ways consistent with their ecological needs. Expand
Olfactory regulation of mosquito-host interactions.
Significant advances in behavioral, physiological and molecular investigations into mosquito host preference are reviewed, with a particular emphasis on studies that have emerged in the post-genomic era that seek to combine these approaches. Expand
Molecular characterization of the Aedes aegypti odorant receptor gene family
The olfactory‐driven blood‐feeding behaviour of female Aedes aegypti mosquitoes is the primary transmission mechanism by which the arboviruses causing dengue and yellow fevers affect over 40 millionExpand
Disruption of Mosquito Olfaction
An improved understanding of the molecular and cellular pathways driving mosquito attraction to humans stands to inform the development of both chemical and genetic strategies to control dengue and malaria. Expand
Functional Characterization of Odorant Receptors in Disease Vector Mosquitoes
The identification and characterization of the first ORco family agonist, VUAA1, is reported, using the Anopheles gambiae co-receptor (AgORco) and other orthologs, which provides a first in class agonist to further research in insect OR signaling. Expand
Insect Odorant Receptors Are Molecular Targets of the Insect Repellent DEET
It is shown that DEET blocks electrophysiological responses of olfactory sensory neurons to attractive odors in Anopheles gambiae and Drosophila melanogaster, and it is concluded thatDEET masks host odor by inhibiting subsets of heteromeric insect odorant receptors that require the OR83b co-receptor. Expand
On the ORigin of smell: odorant receptors in insects
  • R. Benton
  • Biology, Medicine
  • Cellular and Molecular Life Sciences CMLS
  • 2006
Recent revelations of significant structural and functional differences between the Drosophila and mammalian odorant receptor proteins are reviewed and the implications for the understanding of the evolutionary and molecular biology of the insect odorant receptors are discussed. Expand
An Odorant Receptor from the Southern House Mosquito Culex pipiens quinquefasciatus Sensitive to Oviposition Attractants
It is shown that CquiOR2, an antennae-specific OR, which shares high identity with putative orthologs in Anopheles gambiae (AgamOR2) and Aedes aegypti (AaegOR2), is highly sensitive to indole, an oviposition attractant for Cx. Expand
Odorant receptor-mediated sperm activation in disease vector mosquitoes
A subset of odorant receptors showing transcript expression in the testes of the malaria mosquito, Anopheles gambiae, is identified and functional evidence that the broadly conserved insect coreceptor, Orco, mediates flagellar activation in mosquito spermatozoa is provided. Expand
Interactions of Anopheles gambiae Odorant-binding Proteins with a Human-derived Repellent
The crystal structure of A. gambiae OBP1 (OBP1) in the complex it forms with a natural repellent 6-methyl-5-heptene-2-one (6-MH) is presented, and it is found that 6-MH binds to O BP1 at exactly the same site as DEET. Expand


Candidate odorant receptors from the malaria vector mosquito Anopheles gambiae and evidence of down-regulation in response to blood feeding
Four genes encoding candidate odorant receptors from A. gambiae that are selectively expressed in olfactory organs, contain approximately seven transmembrane domains, and show significant similarity to several putative odorant receptor in Drosophila melanogaster are identified. Expand
Olfaction in mosquito-host interactions
Partial table of contents: Vector Insects and Their Control (M. Lehane). Genetics, Ecology and Behaviour of Anophelines (G. Gibson). Odour Plumes and Odour-Mediated Flight in Insects (R. Card?).Expand
G Protein-Coupled Receptors in Anopheles gambiae
This work used bioinformatic approaches to identify 276 G protein–coupled receptors from the Anopheles gambiae genome that are likely to play roles in pathways affecting almost every aspect of the mosquito's life cycle. Expand
Inhibition of host-seeking response and olfactory responsiveness in Anopheles gambiae following blood feeding.
The results show that there was a strong inhibition of host seeking in An. Expand
The olfactory responses of tsetse flies, Glossina spp. (Diptera: Glossinidae), to phenols and urine in the field
In Zimbabwe, catches in traps baited with acetone and 1-octen-3-ol were increased by the addition of the synthetic mixture of eight phenols found in cattle urine to a level equal to or greater than those with natural urine, indicating that the phenols account for essentially all the attractiveness of cattle urine. Expand
Identification of electrophysiologically‐active compounds for the malaria mosquito, Anopheles gambiae, in human sweat extracts
  • A. Cork, K. C. PARK
  • Biology, Medicine
  • Medical and veterinary entomology
  • 1996
Human sweat samples were chemically fractionated into acid and non‐acid components and the major component of sweat was found to be L‐lactic acid which constituted from 1 to 5 mg/ml. Expand
Integrating the Molecular and Cellular Basis of Odor Coding in the Drosophila Antenna
Analysis of a deletion mutant lacking Or22a, along with transgenic rescue experiments, confirms the mapping and demonstrates that an Or gene is required for olfactory function in vivo, and describes the expression of theOr22a and Or22b receptor genes, show localization to dendritic membranes, and find sexual dimorphism. Expand
A Determinant for Directionality of Organelle Transport in Drosophila Embryos
Halo acts as a directionality determinant for embryonic droplet transport and is the first member of a new class of transport regulators in early Drosophila embryos. Expand