Old Yellow Enzyme from Trypanosoma cruzi Exhibits In Vivo Prostaglandin F2α Synthase Activity and Has a Key Role in Parasite Infection and Drug Susceptibility

  title={Old Yellow Enzyme from Trypanosoma cruzi Exhibits In Vivo Prostaglandin F2$\alpha$ Synthase Activity and Has a Key Role in Parasite Infection and Drug Susceptibility},
  author={Florencia D{\'i}az-Viraqu{\'e} and Mar{\'i}a Laura Chiribao and Andrea Trochine and Fabiola Gonz{\'a}lez-Herrera and Christian Castillo and Ana Liempi and Ulrike Kemmerling and Juan Diego Maya and Carlos Robello},
  journal={Frontiers in Immunology},
The discovery that trypanosomatids, unicellular organisms of the order Kinetoplastida, are capable of synthesizing prostaglandins raised questions about the role of these molecules during parasitic infections. Multiple studies indicate that prostaglandins could be related to the infection processes and pathogenesis in trypanosomatids. This work aimed to unveil the role of the prostaglandin F2α synthase TcOYE in the establishment of Trypanosoma cruzi infection, the causative agent of Chagas… Expand
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A Key Role for Old Yellow Enzyme in the Metabolism of Drugs by Trypanosoma cruzi
Interestingly, immunoprecipitation experiments revealed that anti-TcoyE polyclonal antibody abolished major reductase activities of the lysates toward these drugs, identifying TcOYE as a key drug-metabolizing enzyme by which quinone drugs have their mechanism of action. Expand
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Prostaglandin F2α synthase in Trypanosoma cruzi plays critical roles in oxidative stress and susceptibility to benznidazole
Interestingly, parasites overexpressing OYE showed alteration of enzymes associated with oxidative stress protection such as superoxide dismutase A and trypanothione reductase and transfected parasites were more sensitive to drugs, genetic damage and oxidative stress and were less infective than wild-type parasites. Expand
Identification of a Novel Prostaglandin F2α Synthase in Trypanosoma brucei
It is shown that the protozoan parasite T. brucei is involved in PG production and that it produces PGs enzymatically from AA and its metabolite, PGH2, and that TbPGFS is completely distinct from mammalian PGF synthases. Expand
Thromboxane A2 is a key regulator of pathogenesis during Trypanosoma cruzi infection
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An update on the current knowledge of the mechanisms employed by the parasite to gain entry into the host cells and establish persistent infection despite activation of a potent immune response by the host is provided. Expand
Identification, cloning and characterization of an aldo-keto reductase from Trypanosoma cruzi with quinone oxido-reductase activity.
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Early Trypanosoma cruzi Infection Reprograms Human Epithelial Cells
The results raise the possibility that early host cell reprogramming is exploited by the parasite to establish the initial infection and posterior systemic dissemination. Expand
Circulating levels of cyclooxygenase metabolites in experimental Trypanosoma cruzi infections.
A comparative study shows that these mediators could be mainly related to protective mechanisms in the acute phase, but seem not to be involved in its maintenance in the chronic T. cruzi infections. Expand
Aspirin Treatment of Mice Infected with Trypanosoma cruzi and Implications for the Pathogenesis of Chagas Disease
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