Oil Body Formation in Marchantia polymorpha Is Controlled by MpC1HDZ and Serves as a Defense against Arthropod Herbivores

  title={Oil Body Formation in Marchantia polymorpha Is Controlled by MpC1HDZ and Serves as a Defense against Arthropod Herbivores},
  author={Facundo Romani and Elizabeta Bani{\'c} and Stevie N. Florent and Takehiko Kanazawa and Jason Q.D. Goodger and Remco Mentink and Tom Dierschke and Sabine Zachgo and Takashi Ueda and John L. Bowman and Miltos Tsiantis and Javier Moreno},
  journal={Current Biology},
The origin of a terrestrial flora in the Ordovician required adaptation to novel biotic and abiotic stressors. Oil bodies, a synapomorphy of liverworts, accumulate secondary metabolites, but their function and development are poorly understood. Oil bodies of Marchantia polymorpha develop within specialized cells as one single large organelle. Here, we show that a class I homeodomain leucine-zipper (C1HDZ) transcription factor controls the differentiation of oil body cells in two different… Expand
9 Citations
Fungal-Type Terpene Synthases In Marchantia Polymorpha Are Involved In Sesquiterpene Biosynthesis In Oil Body Cells.
The expression of a subset of MpFTPSLs was induced under non-axenic growth on vermiculite, which increased the amounts of sesquiterpenes and number of oil bodies, and suppression of a portion of M pFTPSSs through RNA interference reduced sesQuiterpene accumulation in thalli grown on verMiculite. Expand
The liverwort oil body is formed by redirection of the secretory pathway
It is found that two paralogous syntaxin-1 homologs in the liverwort Marchantia polymorpha are distinctly targeted to forming cell plates and the oil body, suggesting that these structures share some developmental similarity. Expand
DE-ETIOLATED1 has a role in the circadian clock of the liverwort Marchantia polymorpha.
It is found that the circadian rhythm of MpDET1 expression is disrupted in loss-of-function mutants of core clock genes and putative evening-complex genes, suggesting that Mp DET1 has a yet unknown function in the M. polymorpha circadian clock. Expand
Plant evolution driven by interactions with symbiotic and pathogenic microbes
How insights from a range of plant and algal genomes reveal sustained use through evolution of ancient gene modules as well as emergence of lineage-specific specializations is reviewed. Expand
Ties between Stress and Lipid Droplets Pre-date Seeds.
This work proposes an evolutionary scenario in which the generation of LDs arose as a mechanism to mediate general drought and desiccation resilience, and the required protein framework was co-opted by spermatophytes for a seed-specific program. Expand
SARS-CoV-2 fears green: the chlorophyll catabolite Pheophorbide a is a potent antiviral
The results indicate that PheoA displays a remarkable potency and a satisfactory therapeutic index, which together with its previous use in photoactivable cancer therapy in humans suggest that it may be considered as a potential candidate for antiviral therapy against SARS-CoV-2. Expand
Plant Evolution: Divergent Plants, Divergent Functions for C1HDZ Orthologs
  • I. Monte
  • Biology, Medicine
  • Current Biology
  • 2020
The recent functional characterization of the C1HDZ transcription factor in the early-diverging land plant Marchantia polymorpha reveals its role in biotic stress responses, contrary to its orthologs in flowering plants. Expand
A versatile Tn7 transposon-based bioluminescence tagging tool for quantitative and spatial detection of bacteria in plants
A broadly applicable genetic engineering tool for bioluminescence-based quantitative and spatial detection of bacteria in plants and plasmid vectors that have broad host ranges and enable Tn7 transposon-mediated integration of the luxCDABE luciferase operon into a specific genomic location ubiquitously found across bacterial phyla are devised. Expand
A versatile Tn7 transposon-based bioluminescence tagging tool for quantitative and spatial detection of bacteria in plants
Assays using vectors that enable Tn7 transposon-mediated integration of the luxCDABE luciferase operon into a specific genomic location found ubiquitously across bacterial phyla accurately reported the effects of plant immunity and bacterial effectors on bacterial growth, with a dynamic range of four orders of magnitude. Expand


MpTCP1 controls cell proliferation and redox processes in Marchantia polymorpha.
Redox sensitivity of the DNA binding capacity of MpTCP1 proteins provides a mechanism to respond to altered redox conditions and could have contributed to diversification of land plant morphologies and to adaptations to abiotic and biotic challenges, experienced by liverworts during early land plant colonization. Expand
Jasmonate-Related MYC Transcription Factors Are Functionally Conserved in Marchantia polymorpha.
Phenotypic and molecular characterization of loss- and gain-of-function mutants demonstrated that MpMYCs are necessary and sufficient for activating the jasmonate pathway in M. polymorpha, but unlike their Arabidopsis orthologs, do not regulate fertility, suggesting that despite 450 million years of independent evolution, MYCs are functionally conserved between bryophytes and eudicots. Expand
A Simple Auxin Transcriptional Response System Regulates Multiple Morphogenetic Processes in the Liverwort Marchantia polymorpha
The results indicate auxin mediated transcriptional regulation acts as a facilitator of branching, differentiation and growth, rather than acting to determine or specify tissues during the haploid stage of the M. polymorpha life cycle. Expand
Genetic analysis of the liverwort Marchantia polymorpha reveals that R2R3MYB activation of flavonoid production in response to abiotic stress is an ancient character in land plants.
MpMyb14 regulates stress-induced flavonoid production in M. polymorpha, and is essential for red pigmentation, which suggests that R2R3MYB regulated flavonoids production is a conserved character across land plants which arose early during land colonization. Expand
A bHLH Transcription Factor Regulates Bisbibenzyl Biosynthesis in the Liverwort Plagiochasma appendiculatum
In both callus and thallus, the overexpression of PabHLH boosted bisbibenzyl accumulation, while also up-regulating PaPAL, Pa4CL1, PaSTCS1 and two genes encoding P450 cytochromes, and its RNA interference-induced suppression down-regulated the same set of genes and reduced the accumulation of bisb Bibenzyls. Expand
Abscisic acid-induced gene expression in the liverwort Marchantia polymorpha is mediated by evolutionarily conserved promoter elements.
The results demonstrate the presence of ABA-specific response mechanisms mediated by conserved cis-regulatory elements in liverworts, implying that the mechanisms had been acquired in the common ancestors of embryophytes. Expand
Insights into Land Plant Evolution Garnered from the Marchantia polymorpha Genome
Compared with other sequenced land plants, M. polymorpha exhibits low genetic redundancy in most regulatory pathways, with this portion of its genome resembling that predicted for the ancestral land plant. Expand
Abscisic acid induces biosynthesis of bisbibenzyls and tolerance to UV-C in the liverwort Marchantia polymorpha.
Data show that ABA plays a significant role in the induction of bisbibenzyl biosynthesis, which might confer tolerance against UV-C irradiation in M. polymorpha. Expand
Biosynthesis of riccionidins and marchantins is regulated by R2R3-MYB transcription factors in Marchantia polymorpha
The results suggest that the regulatory framework of phenolic metabolism by R2R3-MYB was already established in early land plants. Expand
Molecular Genetic Tools and Techniques for Marchantia polymorpha Research.
The technologies and resources for reverse and forward genetics in M. polymorpha are described, which offer an excellent experimental platform to study the evolution and diversity of regulatory systems in land plants. Expand