Offspring from Oocytes Derived from in Vitro Primordial Germ Cell–like Cells in Mice

  title={Offspring from Oocytes Derived from in Vitro Primordial Germ Cell–like Cells in Mice},
  author={Katsuhiko Hayashi and Sugako Ogushi and Kazuki Kurimoto and So Shimamoto and Hiroshi Ohta and Mitinori Saitou},
  pages={971 - 975}
Artificially Induced Oocytes In mice, male embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) have been shown to differentiate into primordial germ cell–like cells (PGCLCs) in vitro. Upon transplantation into testes, these PGCLCs can form fully functional sperm. Again working in mice, Hayashi et al. (p. 971, published online 4 October) found that female ESCs and iPSCs can also differentiate into PGCLCs, which, when aggregated in reconstituted ovaries, exhibited epigenetic… 

Functional primordial germ cell–like cells from pluripotent stem cells in rats

In vitro–induced rat primordial germ cells undergo spermatogenesis in vivo, enabling the birth of normal offspring, and this system will allow comparative studies and enable broader execution and analysis of in vitro gametogenesis.

Generation of eggs from mouse embryonic stem cells and induced pluripotent stem cells

This method provides an initial step toward reconstitution of the entire process of oogenesis in vitro, which is an integrated process through which an egg acquires the potential for totipotency, a fundamental condition for creating new individuals.

Generation of Mouse Functional Oocytes in Rat by Xeno-Ectopic Transplantation of Primordial Germ Cells1

These studies show that rat/mouse female PGCs and PGC-free gonadal cells can develop and reconstruct ovary-like tissue containing functional oocytes in an ectopic xenogenic microenvironment.

Generation of germ cells in vitro in the era of induced pluripotent stem cells

These findings illustrate not only that iPSCs are developmentally similar to embryonic stem cells (ESCs), but also that somatic cells from adult tissues can produce gametes in vitro, that is, if they are reprogrammed into iPSC.

Aberrant Gene Expression and Sexually Incompatible Genomic Imprinting in Oocytes Derived from XY Mouse Embryonic Stem Cells In Vitro

The results suggest that the developmental dysfunction of the ESC-derived FLSs may be attributable to aberrant gene expression and genomic imprinting, possibly associated with uncertain sex determination in culture.

Human Germline Development from Pluripotent Stem Cells in vitro

A review of the current status in the field of in vitro germ cell production from pluripotent stem cells is presented and how its usefulness could be extended to clinical applications is discussed.

Induction of meiosis by embryonic gonadal somatic cells differentiated from pluripotent stem cells

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Gamete derivation from embryonic stem cells, induced pluripotent stem cells or somatic cell nuclear transfer-derived embryonic stem cells: state of the art.

Several strategies for deriving gametes from PSCs from mice and primates (human and non-human) and their anticipated strengths, challenges and limitations are considered.

Generation of human oogonia from induced pluripotent stem cells in vitro

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Perspectives of germ cell development in vitro in mammals

The requirements for a culture system to generate the germ cell lineage from ESCs/iPSCs are discussed, which would contribute not only to dissection of the biological processes of germ cell development but also to production of unlimited numbers of functional gametes in vitro.



Generation of Functional Oocytes and Spermatids from Fetal Primordial Germ Cells after Ectopic Transplantation in Adult Mice1

The transplanted PGC and gonadal somatic cells constructed testis-like and ovary-like tissues, respectively, under the kidney capsules within 4 wk, demonstrating the remarkable flexibility of PGC development, which can proceed up to the functional gamete stage under spatially and temporally noninnate conditions.

Derivation of Oocytes from Mouse Embryonic Stem Cells

It is shown that mouse embryonic stem cells in culture can develop into oogonia that enter meiosis, recruit adjacent cells to form follicle-like structures, and later develop into blastocysts.

Mouse Offspring Derived from Fetal Ovaries or Reaggregates Which were Cultured and Transplanted into Adult Females

Primordial germ cells (PGCs) and gonia could be promising novel targets and vehicles for manipulation of the mammalian germ line. To make such manipulation a practical possibility, PGCs or gonia must

Development in vitro of mouse oocytes from primordial follicles.

The results reported here clearly show that complete development of oocytes in vitro from the primordial follicle stage is possible and establish the framework for further studies using oocytes from laboratory animals as model systems for the development of Oocytes from humans as well as from animals of agricultural and zoological importance.

Oogenesis: Maturation of mouse fetal germ cells in vitro

This work shows that premeiotic female germ cells derived from mouse fetuses as early as 12.5 days post coitum are able to complete meiosis and genomic imprinting in vitro and that these matured oocytes are highly competent in supporting development to full term after nuclear transfer and in vitro fertilization.

Female mouse germ cells form synchronously dividing cysts.

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Generation of stella‐GFP transgenic mice: A novel tool to study germ cell development

The use of germline‐restricted expression of stella is described, which is currently the best marker gene for PGCs, and two stella‐GFP reporters are generated and it is shown that both transgenes surpass other reporters in terms of timing and specificity of expression in P GCs.

Global hypomethylation of the genome in XX embryonic stem cells

DNA methylation is globally reduced in XX ES cell lines and that this is attributable to the presence of two active X chromosomes, and it is shown that hypomethylation is associated with reduced levels of the de novo DNA methyltransferases Dnmt3a and DnMT3b and that ectopic expression of these factors restores global methylation levels.