Off-target Effects in CRISPR/Cas9-mediated Genome Engineering

  title={Off-target Effects in CRISPR/Cas9-mediated Genome Engineering},
  author={X. Zhang and Louis Y Tee and Xiao-gang Wang and Q. Huang and Shi-hua Yang},
  journal={Molecular Therapy. Nucleic Acids},
  • X. Zhang, Louis Y Tee, +2 authors Shi-hua Yang
  • Published 2015
  • Medicine, Biology
  • Molecular Therapy. Nucleic Acids
  • CRISPR/Cas9 is a versatile genome-editing technology that is widely used for studying the functionality of genetic elements, creating genetically modified organisms as well as preclinical research of genetic disorders. However, the high frequency of off-target activity (≥50%)—RGEN (RNA-guided endonuclease)-induced mutations at sites other than the intended on-target site—is one major concern, especially for therapeutic and clinical applications. Here, we review the basic mechanisms underlying… CONTINUE READING

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    Small molecules enhance CRISPR genome editing in pluripotent stem cells.
    • 275
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    Genetic Screens in Human Cells Using the CRISPR-Cas9 System
    • 1,854
    • Open Access
    Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity
    • 1,754
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