Off-rate screening (ORS) by surface plasmon resonance. An efficient method to kinetically sample hit to lead chemical space from unpurified reaction products.

Abstract

The dissociation rate constant kd (off-rate) is the component of ligand-protein binding with the most significant potential to enhance compound potency. Here we provide theoretical and empirical data to show that this parameter can be determined accurately from unpurified reaction products containing designed test compounds. This screening protocol is… (More)
DOI: 10.1021/jm401848a

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Cite this paper

@article{Murray2014OffrateS, title={Off-rate screening (ORS) by surface plasmon resonance. An efficient method to kinetically sample hit to lead chemical space from unpurified reaction products.}, author={James B Murray and Stephen D Roughley and Natalia B Matassova and Paul A Brough}, journal={Journal of medicinal chemistry}, year={2014}, volume={57 7}, pages={2845-50} }