Off-Target Effect of doublecortin Family shRNA on Neuronal Migration Associated with Endogenous MicroRNA Dysregulation

@article{Baek2014OffTargetEO,
  title={Off-Target Effect of doublecortin Family shRNA on Neuronal Migration Associated with Endogenous MicroRNA Dysregulation},
  author={Seung Tae Baek and G{\'e}raldine Kerjan and Stephanie L Bielas and Ji Eun Lee and Ali G. Fenstermaker and Gaia Novarino and Joseph G Gleeson},
  journal={Neuron},
  year={2014},
  volume={82},
  pages={1255-1262}
}
Acute gene inactivation using short hairpin RNA (shRNA, knockdown) in developing brain is a powerful technique to study genetic function; however, discrepancies between knockdown and knockout murine phenotypes have left unanswered questions. For example, doublecortin (Dcx) knockdown but not knockout shows a neocortical neuronal migration phenotype. Here we report that in utero electroporation of shRNA, but not siRNA or miRNA, to Dcx demonstrates a migration phenotype in Dcx knockouts akin to… CONTINUE READING
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