Of mice and men: human RNA polymerase III promoter U6 is more efficient than its murine homologue for shRNA expression from a lentiviral vector in both human and murine progenitor cells.

@article{Roelz2010OfMA,
  title={Of mice and men: human RNA polymerase III promoter U6 is more efficient than its murine homologue for shRNA expression from a lentiviral vector in both human and murine progenitor cells.},
  author={R Roelz and Ingo H. Pilz and Manuel Mutschler and Heike Luise Pahl},
  journal={Experimental hematology},
  year={2010},
  volume={38 9},
  pages={
          792-7
        }
}
OBJECTIVE RNA interference mediated by transcription of short hairpin RNAs (shRNAs) from lentiviral expression vectors has emerged as an efficient method to effectively and specifically silence gene expression in a vast variety of mammalian cells. shRNA expression is routinely driven by a RNA polymerase III promoter, most often by the U6 promoter. Here we demonstrate that U6 promoter activity-and consequently gene silencing success-differs significantly among species. MATERIALS AND METHODS We… CONTINUE READING
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