Occurrence of nicotinamide adenine dinucleotide phosphatelinked glyoxylate reductase in nonphotosynthetic xylem tissue of perennials.


Xylem extracts of poplar tree contained glyoxylate reductase specific for NADPH. By isoelectric focusing in the pH ranges 3.5 to 10 or 4 to 6, the enzyme exhibited a single peak of activity at pH 5.4. The enzyme showed essentially no activity toward hydroxypyruvate, pyruvate, or NADH. The reaction was optimal at pH 6.0 in phosphate buffer and the activity… (More)


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