Occurrence of bacterial black shoot disease of European pear in Yamagata Prefecture

@article{Mizuno2009OccurrenceOB,
  title={Occurrence of bacterial black shoot disease of European pear in Yamagata Prefecture},
  author={Akifumi Mizuno and Takanori Tsukamoto and Yoshiaki Shimizu and Hitoshi Ooya and Takayuki Matsuura and Norihiko Saito and Shigeyoshi Sato and Shigemi Kikuchi and Tsuneyasu Uzuki and Kouji Azegami},
  journal={Journal of General Plant Pathology},
  year={2009},
  volume={76},
  pages={43-51}
}
Black lesions on shoots of European pear trees observed in an orchard in Yamagata Prefecture in May 2007 were suspected to be caused by a bacterial pathogen. The surface of the colonies isolated on a high sucrose medium did not have the crater morphology that is characteristic of E. amylovora bvs. 1–3, and a specific DNA fragment was amplified from the isolates in the PCR using the EprpoD primer set. The partial sequences of the 16S rRNA gene placed the isolates in the genus Erwinia. The… CONTINUE READING

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