Occurrence of Giardia intestinalis and Cryptosporidium sp. in wastewater samples from São Paulo State, Brazil, and Lima, Peru.

@article{UlloaStanojlovi2016OccurrenceOG,
  title={Occurrence of Giardia intestinalis and Cryptosporidium sp. in wastewater samples from S{\~a}o Paulo State, Brazil, and Lima, Peru.},
  author={Francisco Miroslav Ulloa-Stanojlovi{\'c} and Bruna Andrade Aguiar and Luis M. Jara and Maria In{\^e}s Zanoli Sato and Juana Arzola Guerrero and Elayse Maria Hachich and Glavur Rog{\'e}rio Matt{\'e} and Milena Dropa and Maria Helena Matt{\'e} and Ronalda Silva de Ara{\'u}jo},
  journal={Environmental science and pollution research international},
  year={2016},
  volume={23 21},
  pages={
          22197-22205
        }
}
The objectives of the study were to detect and genotype Cryptosporidium spp. and Giardia intestinalis in wastewater samples obtained from five cities with high transit of people in the State of São Paulo, Brazil, and at the entrance of a Wastewater Treatment Plant (WWTP) in Lima, Peru. Samples were collected and concentrated by centrifugation. The genomic DNA was extracted for molecular characterization by nested PCR for Cryptosporidium and double nested PCR for Giardia, followed by sequencing… CONTINUE READING
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