Occurrence and characterization of microsomal cytochrome P-450 in several vertebrate and insect species.

@article{Kulkarni1976OccurrenceAC,
  title={Occurrence and characterization of microsomal cytochrome P-450 in several vertebrate and insect species.},
  author={A. Kulkarni and E. Smith and E. Hodgson},
  journal={Comparative biochemistry and physiology. B, Comparative biochemistry},
  year={1976},
  volume={54 4},
  pages={
          509-13
        }
}
Abstract 1. 1. Microsomal cytochrome P-450s from 19 species, including mammals, birds, fish and insects, were characterized by optical difference spectra formed by this interaction with a number of ligands. 2. 2. These include: carbon monoxide; benzphetamine (type I); pyridine (type II); n-octylamine (type II); ethyl isocyanide (type III). 3. 3. In addition the following enzyme activities were determined: O-demethylation of p-nitroanisole; NADPH oxidation; NADPH-cytochrome reductase (E.C. 1.6.2… Expand
Cytochrome P-450 in insects
Abstract Soluble cytochrome P -450 prepared from the microsomal fraction of abdomen homogenates of an insecticide resistant strain (Rutgers) and a susceptible strain (NAIDM) of the house fly, MuscaExpand
Hepatic microsomes from freshwater fish--I. In vitro cytochrome P-450 chemical interactions.
  • D. L. Fabacher
  • Biology, Medicine
  • Comparative biochemistry and physiology. C: Comparative pharmacology
  • 1982
TLDR
Spectral data suggest that hepatic microsomal cytochrome P-450 of freshwater fish exists in different forms, since the spectral size of chemical interactions as related to the carbon monoxide spectrum and the ratio of type II to type I binding were not alike. Expand
Initial characterization of the hepatic microsomal cytochrome P-450-system of the Piked Whale (Minke) Balaenoptera acutorostrata
TLDR
Components of the microsomal cytochrome P -450- system were measured in liver samples from several female, one male and two foetal piked whales and in vitro metabolism of phenanthrene showed a clear preference for attack at the 9,10-position by adult liver microsomes, with large individual variations. Expand
An unusual effect of N-octylamine on the cytochrome b5 spectrum of insect and mammalian microsomes.
TLDR
Microsomes from rat and mouse liver, but not those from housefly abdomens, exhibit similar unusual spectral interactions with n-octylamine when supplemented with the soluble factor from the hornworm. Expand
Cytochrome P-450 monooxygenases in insects.
  • M. Ronis, E. Hodgson
  • Biology, Medicine
  • Xenobiotica; the fate of foreign compounds in biological systems
  • 1989
TLDR
The genetics, purification and properties of cytochrome P-450 isozymes of the house fly (Musca domestica) are discussed in relation to their role in resistance to xenobiotics and with reference to polyphagy. Expand
Effect of n-octylamine on the reduction of mammalian hepatic microsomal cytochrome b5.
TLDR
The experiments reported here demonstrate that low concentrations of n-octylamine cause an increased level of cytochrome b5 reduction by mouse hepatic microsomes and also delays its reoxidation, and suggest that the enzyme(s) involved in the endogenous reduction of NAD(P)+ is not the microsomal alcohol dehydrogenase. Expand
Characterization of Cytochrome P-450 in Studies of Insecticide Resistance
TLDR
In one or two species of insect, such as the house fly, Musca domestica, and the southern armyworm, Spodopteva evidania, P-450 and its related enzymes have been described in some detail. Expand
The occurrence of cytochrome P-450 and aryl hydrocarbon hydroxylase activity in Drosophila melanogaster microsomes, and the importance of this metabolizing capacity for the screening of carcinogenic and mutagenic properties of foreign compounds.
TLDR
Findings provide the basis for further investigations, by which a better understanding of the metabolizing capacity of Drosophila can make this species even more meaningful for the screening of pre-carcinogens and indirectly acting mutagens. Expand
the metabolism of drugs and carcinogens in isolated subcellular fractions of Drosophila melanogaster. II. Enzyme induction and metabolism of benzo[a]pyrene.
TLDR
Variability in the induction response to the various pretreatment was observed among the three strains, whereas the activities of epoxide hydrolase and glutathione-S-transferase in control larvae were comparable in the different strains,Whereas the content of cytochrome P-450 and BP monooxygenase activity was higher in the Hikone R strain. Expand
Oxidative and conjugative metabolism of xenobiotics by livers of cattle, sheep, swine and rats.
TLDR
Results show that hepatic enzymatic capabilities toward xenobiotics do not parallel phylogenetic classifications, thus strengthening the view that most of the comparative data available at present is more descriptive than predictive of relationships among species. Expand
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