Occurrence and biosynthesis of 3-mercaptopropionic acid in Methanocaldococcus jannaschii.

  title={Occurrence and biosynthesis of 3-mercaptopropionic acid in Methanocaldococcus jannaschii.},
  author={Kylie D. Allen and Robert H White},
  journal={FEMS microbiology letters},
  volume={363 19}
In a non-targeted analysis of thiol-containing compounds in the hyperthermophilic methanogen Methanocaldococcus jannaschii, we discovered three unexpected metabolites: 3-mercaptopropionic acid (MPA), 2-hydroxy-4-mercaptobutyric acid (HMBA) and 4-mercapto-2-oxobutyric acid (MOB). HMBA and MOB have never been reported as natural products, while MPA is highly prevalent in aquatic environments as a result of biotic and abiotic processing of sulfur-containing compounds. This report provides evidence… Expand
Expression, Purification, and Characterization of (R)-Sulfolactate Dehydrogenase (ComC) from the Rumen Methanogen Methanobrevibacter millerae SM9
The critical role of ComC in two separate cofactor pathways makes this enzyme a potential means of developing methanogen-specific inhibitors for controlling ruminant methane emissions which are increasingly being recognized as contributing to climate change. Expand
Identification and Biosynthesis of 1-Mercaptoethanesulfonic Acid (1-MES), an Analogue of Coenzyme M, Found Widely in the Methanogenic Archaea.
1-MES is formed enzymatically when a solution of vinylsulfonic acid and sulfide were exposed to ultraviolet light, consistent with the abstraction of a C-2 deuterium of the HSCoM, followed by a radical rearrangement in which the sulfonic acid moves to position C-1. Expand
Promiscuity of methionine salvage pathway enzymes in Methanocaldococcus jannaschii.
Data suggest that MTIP and MTRI may function in a novel pathway for recycling the 5'-deoxyadenosine moiety of SAM in M. jannaschii, and may enable biosynthesis of 6-deoxy-5-ketofructose 1-phosphate (DKFP), an essential intermediate in aromatic amino acid biosynthesis. Expand
Identification of a Redox Active Thioquinoxalinol Sulfate Compound Produced by an Anaerobic Methane-Oxidizing Microbial Consortium
Thioquinoxalinol compounds, which represent completely new structures in biochemistry, may be involved in electron transport processes within and/or between ANME-1 and sulfate-reducing bacteria, may serve protective roles by reacting with toxic compounds such as hydrogen sulfide, or may transport sulfate as a sulfate ester into the sulfATE-red reducing bacteria. Expand
Identification and biosynthesis of 2-(1H-imidazol-5-yl) ethan-1-ol (histaminol) in methanogenic archaea.
This work describes histaminol production and secretion into the culture broth by the methanogen Methanococcus maripaludis S2 as well as a number of other methanogens, which is the first identification of this compound as a natural product in meethanogens. Expand
Sulfur Assimilation and Trafficking in Methanogens
Methanogens are ancient obligate anaerobes, originating in an early Earth period before oxygen accumulated in the atmosphere and oceans. Unlike aerobic cells, all methanogens that persist in highlyExpand
Editorial: 115 years of sulfur microbiology.
  • R. Boden
  • Chemistry, Medicine
  • FEMS microbiology letters
  • 2017


Identification of an Archaeal 2-Hydroxy Acid Dehydrogenase Catalyzing Reactions Involved in Coenzyme Biosynthesis in Methanoarchaea
  • M. Graupner, Huimin Xu, Robert H. White
  • Medicine, Biology
  • Journal of bacteriology
  • 2000
It is concluded that the MJ1425-encoded enzyme is likely to be involved in the biosynthesis of both coenzyme M and methanopterin. Expand
3-Mercaptopropionate Dioxygenase, a Cysteine Dioxygenase Homologue, Catalyzes the Initial Step of 3-Mercaptopropionate Catabolism in the 3,3-Thiodipropionic Acid-degrading Bacterium Variovorax paradoxus*
The hitherto unknown catabolism of 3,3-thiodipropionic acid was elucidated to engineer novel and improved polythioester biosynthesis pathways in the future. Expand
L-Aspartate semialdehyde and a 6-deoxy-5-ketohexose 1-phosphate are the precursors to the aromatic amino acids in Methanocaldococcus jannaschii.
It is reported that 6-deoxy-5-ketofructose 1-phosphate and l-aspartate semialdehyde are precursors to DHQ, consistent with the remaining steps and genes in the pathway being the same as in the established shikimate pathway. Expand
The biosynthesis of cysteine and homocysteine in Methanococcus jannaschii.
The pathway for the biosynthesis of cysteine and homocysteine in Methanococcus jannaschii has been examined using a gas chromatography-mass spectrometry (GC-MS) stable isotope dilution method toExpand
Discovery of Multiple Modified F430 Coenzymes in Methanogens and Anaerobic Methanotrophic Archaea Suggests Possible New Roles for F430 in Nature
Liquid chromatography–high-resolution mass spectrometry is used to identify several new structural variants of F430 in the cell extracts of selected methanogens and ANME, indicating that F430-related molecules have additional functions in nature and will inspire further research to determine the biochemical role(s) of these variants and the pathways involved in their biosynthesis. Expand
Novel thiols of prokaryotes.
  • R. C. Fahey
  • Chemistry, Medicine
  • Annual review of microbiology
  • 2001
Novel thiols found in those bacteria lacking glutathione are included, including glutathion amide and its perthiol, produced by phototrophic purple sulfur bacteria and apparently involved in their sulfide metabolism. Expand
Biotransformations of organosulphur compounds in sediments via 3-mercaptopropionate
3-Mercaptopropionate (3-MPA) is a major organic sulphur compound (thiol) recently detected in the anoxic pore waters of coastal marine sediments1. A chemical mechanism for the formation of 3-MPA hasExpand
Structure of component B (7-mercaptoheptanoylthreonine phosphate) of the methylcoenzyme M methylreductase system of Methanobacterium thermoautotrophicum.
Component B, the heat-stable low-molecular-weight cofactor required for methane production by dialyzed cell-free extracts of Methanobacterium thermoautotrophicum, has been purified to homogeneity andExpand
The anabolic pyruvate oxidoreductase from Methanococcus maripaludis
It is proposed that PorE and PorF are components of a specialized system required to transfer low-potential electrons for pyruvate biosynthesis. Expand
The first examples of (S)-2-hydroxyacid dehydrogenases catalyzing the transfer of the pro-4S hydrogen of NADH are found in the archaea.
It is reported that recombinantly generated (S)-2-hydroxyacid dehydrogenases present in the methanoarchaea Methanococcus jannaschii and Methanothermus fervidus use the pro-4S hydrogen of NADH to reduce a series of 2-oxoacids to the corresponding (S-2-Hydroxyacids. Expand