Occurrence and activation of Ca2+/calmodulin-dependent protein kinase II and its endogenous substrates in bovine adrenal medullary cells.

@article{Yanagihara1994OccurrenceAA,
  title={Occurrence and activation of Ca2+/calmodulin-dependent protein kinase II and its endogenous substrates in bovine adrenal medullary cells.},
  author={Nobuyuki Yanagihara and Yumiko Toyohira and Hideki Yamamoto and Yasuhiro Ohta and Masato Tsutsui and Eishichi Miyamoto and Futoshi Izumi},
  journal={Molecular pharmacology},
  year={1994},
  volume={46 3},
  pages={423-30}
}
We investigated the presence of and the endogenous substrates for Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) in cultured bovine adrenal medullary cells. By a series of chromatographic steps using DEAE-cellulose, calmodulin affinity, and Sephacryl S-300 columns, we partially purified two CaM kinases (peaks I and III) and one calmodulin-binding protein (peak II). Both of the kinases (peaks I and III) showed broad substrate specificities. Peak I, but not peak III, was… CONTINUE READING

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