Occupancy of the Chromophore Binding Site of Opsin Activates Visual Transduction in Rod Photoreceptors

@article{Kefalov1999OccupancyOT,
  title={Occupancy of the Chromophore Binding Site of Opsin Activates Visual  Transduction in Rod Photoreceptors },
  author={Vladimir J Kefalov and M. Carter Cornwall and Rosalie K. Crouch},
  journal={The Journal of General Physiology},
  year={1999},
  volume={113},
  pages={491 - 503}
}
The retinal analogue beta-ionone was used to investigate possible physiological effects of the noncovalent interaction between rod opsin and its chromophore 11-cis retinal. Isolated salamander rod photoreceptors were exposed to bright light that bleached a significant fraction of their pigment, were allowed to recover to a steady state, and then were exposed to beta-ionone. Our experiments show that in bleach-adapted rods beta-ionone causes a decrease in light sensitivity and dark current and… CONTINUE READING