Obtusifoliol 14alpha-demethylase (CYP51) antisense Arabidopsis shows slow growth and long life.

@article{Kushiro2001Obtusifoliol1,
  title={Obtusifoliol 14alpha-demethylase (CYP51) antisense Arabidopsis shows slow growth and long life.},
  author={Masayo Kushiro and Toshitsugu Nakano and Kimiyoshi Sato and Kazutoshi Yamagishi and Tadao Asami and Atsushi Nakano and Suguru Takatsuto and Shozo Fujioka and Yutaka Ebizuka and Shintaro Yoshida},
  journal={Biochemical and biophysical research communications},
  year={2001},
  volume={285 1},
  pages={
          98-104
        }
}
Obtusifoliol 14alpha-demethylase is a plant orthologue of sterol 14alpha-demethylase (CYP51) essential in sterol biosynthesis. We have prepared CYP51 antisense Arabidopsis in order to shed light on the sterol and steroid hormone biosynthesis in plants. Arabidopsis putative CYP51 cDNA (AtCYP51) was obtained from Arabidopsis expressed sequence tag (EST) library and its function was examined in a yeast lanosterol 14alpha-demethylase (Erg11) deficient mutant. A recombinant AtCYP51 protein fused… CONTINUE READING
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