Obtaining information about protein secondary structures in aqueous solution using Fourier transform IR spectroscopy

@article{Yang2015ObtainingIA,
  title={Obtaining information about protein secondary structures in aqueous solution using Fourier transform IR spectroscopy},
  author={Huayan Yang and Shouning Yang and Jilie Kong and Aichun Dong and Shaoning Yu},
  journal={Nature Protocols},
  year={2015},
  volume={10},
  pages={382-396}
}
Fourier transform IR (FTIR) spectroscopy is a nondestructive technique for structural characterization of proteins and polypeptides. The IR spectral data of polymers are usually interpreted in terms of the vibrations of a structural repeat. The repeat units in proteins give rise to nine characteristic IR absorption bands (amides A, B and I–VII). Amide I bands (1,700–1,600 cm−1) are the most prominent and sensitive vibrational bands of the protein backbone, and they relate to protein secondary… CONTINUE READING
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