Observations on the ultrastructure and viability of the cystic stage of Blastocystis hominis from human feces

@article{Moe2009ObservationsOT,
  title={Observations on the ultrastructure and viability of the cystic stage of Blastocystis hominis from human feces},
  author={K. T. Moe and M. Singh and Josephine Howe and L. C. Ho and S. W. Tan and G. C. Ng and Xiang Qun Chen and E. H. Yap},
  journal={Parasitology Research},
  year={2009},
  volume={82},
  pages={439-444}
}
This report describes the ultrastructure and viability of cysts of Blastocystis hominis from feces of infected patients. The cysts were round to ovoid, measured 2-5 μm in size, and contained a condensed cytoplasm that had vacuoles of varying sizes, four nuclei, and as many as six cristate mitochondria. The cell wall was rather electron-lucent. Surprisingly, chromatoid-like structures were found in the cytoplasm and nucleus of some of the cysts. These have not previously been reported in… Expand
In vivo encystation of Blastocystis hominis
TLDR
The study confirms the irregular shedding phenomenon of the organism previously reported, and for the first time, records sequential changes in encystation in stools collected over a time period. Expand
Morphology of Human and Animal Blastocystis Isolates with Special Reference to Reproductive Modes
TLDR
The nuclei in vacuolar and granular forms can be clearly seen when the cells are stained with DAPI or Giemsa, and Binary fission is frequently observed in fresh fecal and cultured samples. Expand
Ultrastuctural Intestinal Pathology Induced by Human Blastocystis in Experimentally Infected Mice
Background: Blastocystis spp. is a single-celled anaerobic enteric parasite that inhabits the lower gastrointestinal tract of humans and many animals. This emerging parasite with a worldwideExpand
Romancing Blastocystis: A 20-Year Affair
This review captures the surrounding enigmatic aspects of Blastocystis sp. perhaps one of the most fascinating organisms seen under the microscope and highlights the pioneering findings fromExpand
Granular Formation during Apoptosis in Blastocystis sp. Exposed to Metronidazole (MTZ)
TLDR
The findings of this study suggest that granular formation during apoptosis is a self-regulatory mechanism to produce higher number of viable cells in response to treatment when developing drugs against the emerging Blastocystis sp. Expand
New Insights on Classification, Identification, and Clinical Relevance of Blastocystis spp
  • K. Tan
  • Biology, Medicine
  • Clinical Microbiology Reviews
  • 2008
TLDR
Accumulating epidemiological, in vivo, and in vitro data strongly suggest that Blastocystis is a pathogen, and a suggestion that previously conflicting observations on the pathogenesis of BlastocyStis are due to pathogenic and nonpathogenic genotypes is suggested. Expand
Blastocystis, an unrecognized parasite: an overview of pathogenesis and diagnosis
Blastocystis sp. is among the few enteric parasites with a prevalence that often exceeds 5% in the general population of industrialized countries and can reach 30–60% in developing countries. ThisExpand
Current status of Blastocystis: A personal view.
TLDR
Some of the recent developments and advances in Blastocystis research are summarized, including updates on diagnostic methods, molecular epidemiology, genetic diversity, host specificity, clinical significance, taxonomy, and genomics, as the authors are now in the microbiome era. Expand
THE RELEVANCE OF THE DEFINITION OF BLASTOCYSTIS SPP. IN ENVIRONMENTAL OBJECTS AS POTENTIAL RISK FACTORS FOR THE OCCURRENCE OF PROTOZOAL HUMAN INFECTION
TLDR
The need to detect blastocysts in environmental objects (water, soil, surfaces) as potential risk factors for the occurrence of a protozoal infection is substantiated. Expand
Molecular epidemiology of Blastocystis infections
TLDR
The first reports of Blastocystis in the elephant, giraffe, Javan lutung, quokka, southern hairy nosed wombat and western grey kangaroo are described. Expand
...
1
2
3
4
...

References

SHOWING 1-10 OF 19 REFERENCES
Ultrastructure of Blastocystis hominis cysts.
Blastocystis hominis cysts were concentrated from human faeces by repeated washing in distilled water and centrifugation on Ficoll-Paque. This procedure gave a concentrated suspension of cystsExpand
Ultrastructure ofBlastocystis hominis cysts
Blastocystis hominis cysts were concentrated from human faeces by repeated washing in distilled water and centrifugation on Ficoll-Paque. This procedure gave a concentrated suspension of cystsExpand
A concentration technique for obtaining viable cysts of blastocystis hominis from faeces.
TLDR
The concentration technique described in this paper separates the cystic phase of Blastocystis hominisis from the non-cystic stages, there by enabling its easy recognition in faecal samples. Expand
Ultrastructure of Blastocystis hominis in human stool samples.
TLDR
Results suggest that the vacuolated form of Blastocystis hominis may be an artefact of culture conditions, and that the form of B. hominis present in the gastrointestinal tract is avacuolar. Expand
Elucidation of the life cycle of the intestinal protozoanBlastocystis hominis
TLDR
It is suggested that the thin-walled cysts in Blastocystis are autoinfectious, leading to multiplication of the organism in the intestinal tract, and a life cycle forB. Expand
A cyst-like stage of Blastocystis hominis.
TLDR
A cyst-like form of Blastocystis hominis is described in stools and in culture that can withstand adverse environmental conditions and is infective to a new host remain to be determined. Expand
Morphology of Blastocystis sp. isolated from circus animals.
Blastocystis sp. is reported for the first time from faecal samples collected from a camel, a llama, a highland bull and a lion in a travelling circus. Fresh faecal specimens were examined by lightExpand
Ultrastructural changes during in vitro encystment ofBlastocystis hominis
TLDR
The morphological changes occurring in Blastocystis hominis at different time points following in vitro encystment were studied by electron microscopy and the amoebic form appeared to be an intermediate stage between the vacuolar form and the pre-cystic form. Expand
Bacteria-like endosymbionts in Blastocystis sp.
Bacteria-like endosymbionts were found in vacuolar cells and cysts of Blastocystis sp. from duck and monkey faecal material. The organisms ultrastructurally resembled Gram-negative bacilli, and wereExpand
Electron microscopy of surface structures ofBlastocystis sp. from different hosts
Samples ofBlastocystis sp. obtained from humans, monkeys, pigs and chickens were examined by scanning electron microscopy and transmission electron microscopy to compare surface structures. TheExpand
...
1
2
...