Observations on the morphology of pronuclei and nucleoli in human zygotes and implications for cryopreservation.

@article{Wright1990ObservationsOT,
  title={Observations on the morphology of pronuclei and nucleoli in human zygotes and implications for cryopreservation.},
  author={Graham L Wright and Sharon R. Wiker and Carlene W. Elsner and Hilton Isadore Kort and Joe B. Massey and David Andrew Mitchell and Andrew A. Toledo and Jacques Cohen},
  journal={Human reproduction},
  year={1990},
  volume={5 1},
  pages={109-15}
}
The effects of cryopreservation on human zygotes at various stages between the appearance of pronuclei and their close association were investigated. Pronuclear zygotes (n = 233) from 101 patients were frozen using propanediol 21-35 h following egg collection. The incidence of implantation of thawed pronuclear zygotes frozen 29-35 h following oocyte collection was significantly higher than that of younger pronuclear zygotes (28 versus 10%, respectively). Zygote age did not affect cell survival… CONTINUE READING

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