Observations on the Influence of the Climate of Canada in Preventing the Development and Stopping the Progress of Physical Symptoms

@inproceedings{Orton1969ObservationsOT,
  title={Observations on the Influence of the Climate of Canada in Preventing the Development and Stopping the Progress of Physical Symptoms},
  author={John W. Orton},
  booktitle={The Journal of cell biology},
  year={1969}
}
Using Chinese hamster cells in culture, we have measured the effectiveness of actinomycin D to suppress division as a function of the position, or age, of a cell in its growth cycle. Cells were first exposed to millimolar concentrations of hydroxyurea in order to produce a synchronized population just before the onset of DNA synthesis. Thereafter, the survival response after 30 min exposures to actinomycin D was measured. Cells become resistant as they enter the S phase and then sensitive again… CONTINUE READING

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