Observational clinical follow-up of oocyte cryopreservation using a slow-freezing method with 1,2-propanediol plus sucrose followed by ICSI.

@article{Chen2005ObservationalCF,
  title={Observational clinical follow-up of oocyte cryopreservation using a slow-freezing method with 1,2-propanediol plus sucrose followed by ICSI.},
  author={Shee-Uan Chen and Y N Lien and Hsin-Fu Chen and Li-Jung Chang and Yi-Yi Tsai and Y Yang},
  journal={Human reproduction},
  year={2005},
  volume={20 7},
  pages={1975-80}
}
BACKGROUND The value of oocyte cryopreservation remains controversial. Two major problems exist: poor survival and injury to the oocyte meiotic spindle after freezing and thawing. METHODS For slow oocyte cryopreservation, we used 1.5 mol/l 1,2-propanediol and 0.3 mol/l sucrose. We waited 3 h after thawing for possible recovery of the meiotic spindles before performing ICSI. RESULTS Forty-three women undergoing IVF or ICSI cycles cryopreserved some or all of their harvested oocytes; of these… CONTINUE READING

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