Observation of topical catalysis by sphingomyelinase coupled to microspheres.

Abstract

Sphingomyelinase, SMase (EC 3.1.4.12), was coupled onto amino-derivatized acrylate microspheres and was shown to retain its catalytic activity. The immobilized enzyme allows one to carry out topical enzymatic reaction in a controlled manner. Accordingly, these spheres were held with a micropipet and using micromanipulator brought into contact with a giant… (More)

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@article{Nurminen2002ObservationOT, title={Observation of topical catalysis by sphingomyelinase coupled to microspheres.}, author={Tuula A Nurminen and Juha Holopainen and Hongxia Zhao and Paavo K. J. Kinnunen}, journal={Journal of the American Chemical Society}, year={2002}, volume={124 41}, pages={12129-34} }