Observation of through-hydrogen-bond 2hJHC' in a perdeuterated protein.

  title={Observation of through-hydrogen-bond 2hJHC' in a perdeuterated protein.},
  author={Florence Cordier and Marco Rogowski and Stephan Grzesiek and Adriaan Bax},
  journal={Journal of magnetic resonance},
  volume={140 2},
It is demonstrated that J connectivity between amide protons and hydrogen-bond-accepting carbonyl carbons can be observed in perdeuterated human ubiquitin. A selective pulse scheme is used to detect these small 2hJHC' interactions in the presence of the much larger through-covalent-bond 2JHC' and 3JHC' couplings. The ratio of the observed through-H-bond correlation intensity and the 2JHC' connectivity observed in a reference spectrum indicates 2hJHC' values of ca. 0.4-0.6 Hz, which are only… 

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