Observation of spider silk by femtosecond pulse laser second harmonic generation microscopy

  title={Observation of spider silk by femtosecond pulse laser second harmonic generation microscopy},
  author={Yue Zhao and Yanrong Li and Khuat Thi Thu Hien and Goro Mizutani and Harvey Rutt},
  journal={Surface and Interface Analysis},
  pages={56 - 60}
An asymmetric β‐sheet structure of spider silk is said to induce optical second harmonic generation. In this paper, using an in‐house nonscanning type femtosecond pulse laser second harmonic generation microscope, we characterized the behavior of the β‐sheet of spider silk under an applied external force. The orientation of the β‐sheets was more unidirectional when the silk was extended. One of the origins of the high mechanical strength of the dragline is suggested to be the physical… 

Sum frequency generation spectroscopy of the attachment disc of a spider.

Sum frequency generation spectroscopy study of the attachment disc of spider's pyriform silk

The pyriform silk molecular orientation of the attachment disc of a spider was studied using infrared-visible vibrational sum frequency generation (SFG) spectroscopy. When a spider secretes

Natural Spider Silk Nanofibrils Produced by Assembling Molecules or Disassembling Fibers

The complete mechanical decomposition of natural silk fibers from the golden silk orb-weaver Trichonephila clavipes is reported, breaking down into ≈10 nm-diameter nanofibrils, the material’s apparent fundamental building blocks.

Spider silk: A natural marvel of mechanical and structural strength

The spider silk fibers have unique high performance properties that make it a desirable model for artificial fibers and its performance under benign conditions has important implications for



Second-order nonlinear optical microscopy of spider silk

Asymmetric $$\upbeta $$β-sheet protein structures in spider silk should induce nonlinear optical interaction such as second harmonic generation (SHG) which is experimentally observed for a radial

Second-harmonic microscopy of biological tissue.

The data show that the currently unexplained macroscopic polar order of this classic representative of connective tissue is due both to a coherent network containing a large number of fine, polar, filamentlike structures that permeate the entire tendon volume and to a small number of intensely polar surface patches.

Molecular Orientation and Two-Component Nature of the Crystalline Fraction of Spider Dragline Silk

Solid-state 2H nuclear magnetic resonance data from unoriented, oriented, and supercontracted fibers suggest that it will be necessary to control the secondary structure of individual polymer molecules in order to obtain optimum properties in bio-inspired polymers.

Molecular and nanostructural mechanisms of deformation, strength and toughness of spider silk fibrils.

This model develops the first spider silk mesoscale model, bridging the scales from Angstroms to tens to potentially hundreds of nanometers, and demonstrates that the specific nanoscale combination of a crystalline phase and a semiamorphous matrix is crucial to achieve the unique properties of silks.

Toward spinning artificial spider silk.

Recent progress has unraveled the molecular mechanisms of the spidroin N- and C-terminal nonrepetitive domains (NTs and CTs) and revealed the pH and ion gradients in spiders' silk glands, clarifying how spIDroin solubility is maintained and how silk is formed in a fraction of a second.

Liquid crystalline spinning of spider silk

Successful copying of the spider's internal processing and precise control over protein folding, combined with knowledge of the gene sequences of its spinning dopes, could permit industrial production of silk-based fibres with unique properties under benign conditions.

Mechanical Properties of Spider Silk.

Threads from spiders may be one of the best structural materials produced by nature. They are used to capture and wrap insects. Less attension has been paid to their mechanical properties of spider

The mechanical design of spider silks: from fibroin sequence to mechanical function.

Comparison of MA silks from Araneus diadematus and Nephila clavipes shows variation in fibroin sequence and properties between spider species provides the opportunity to investigate the design of these remarkable biomaterials.