Observation of spider silk by femtosecond pulse laser second harmonic generation microscopy

@article{Zhao2018ObservationOS,
  title={Observation of spider silk by femtosecond pulse laser second harmonic generation microscopy},
  author={Yue Zhao and Yanrong Li and Khuat Thi Thu Hien and Goro Mizutani and Harvey Rutt},
  journal={Surface and Interface Analysis},
  year={2018},
  volume={51},
  pages={56 - 60}
}
An asymmetric β‐sheet structure of spider silk is said to induce optical second harmonic generation. In this paper, using an in‐house nonscanning type femtosecond pulse laser second harmonic generation microscope, we characterized the behavior of the β‐sheet of spider silk under an applied external force. The orientation of the β‐sheets was more unidirectional when the silk was extended. One of the origins of the high mechanical strength of the dragline is suggested to be the physical… 
View on Wiley
View With Semantic Reader
5 Citations
Background Citations
1

5 Citations

Sum frequency generation spectroscopy of the attachment disc of a spider.
Sum frequency generation spectroscopy study of the attachment disc of spider's pyriform silk
The pyriform silk molecular orientation of the attachment disc of a spider was studied using infrared-visible vibrational sum frequency generation (SFG) spectroscopy. When a spider secretes…
Natural Spider Silk Nanofibrils Produced by Assembling Molecules or Disassembling Fibers
TLDR
The complete mechanical decomposition of natural silk fibers from the golden silk orb-weaver Trichonephila clavipes is reported, breaking down into ≈10 nm-diameter nanofibrils, the material’s apparent fundamental building blocks.
Spider silk: A natural marvel of mechanical and structural strength
The spider silk fibers have unique high performance properties that make it a desirable model for artificial fibers and its performance under benign conditions has important implications for…
Observation of spider silk by femtosecond pulse laser second harmonic generation microscopy

References

SHOWING 1-10 OF 40 REFERENCES
Second-order nonlinear optical microscopy of spider silk
Asymmetric $$\upbeta $$β-sheet protein structures in spider silk should induce nonlinear optical interaction such as second harmonic generation (SHG) which is experimentally observed for a radial…
Second-harmonic microscopy of biological tissue.
TLDR
The data show that the currently unexplained macroscopic polar order of this classic representative of connective tissue is due both to a coherent network containing a large number of fine, polar, filamentlike structures that permeate the entire tendon volume and to a small number of intensely polar surface patches.
Molecular Orientation and Two-Component Nature of the Crystalline Fraction of Spider Dragline Silk
TLDR
Solid-state 2H nuclear magnetic resonance data from unoriented, oriented, and supercontracted fibers suggest that it will be necessary to control the secondary structure of individual polymer molecules in order to obtain optimum properties in bio-inspired polymers.
Molecular and nanostructural mechanisms of deformation, strength and toughness of spider silk fibrils.
TLDR
This model develops the first spider silk mesoscale model, bridging the scales from Angstroms to tens to potentially hundreds of nanometers, and demonstrates that the specific nanoscale combination of a crystalline phase and a semiamorphous matrix is crucial to achieve the unique properties of silks.
Liquid crystals and flow elongation in a spider's silk production line
  • D. Knight, F. Vollrath
  • Materials Science
    Proceedings of the Royal Society of London. Series B: Biological Sciences
  • 1999
Our observations on whole mounted major ampullate silk glands suggested that the thread is drawn from a hyperbolic die using a pre–orientated lyotropic liquid crystalline feedstock. Polarizing…
Toward spinning artificial spider silk.
TLDR
Recent progress has unraveled the molecular mechanisms of the spidroin N- and C-terminal nonrepetitive domains (NTs and CTs) and revealed the pH and ion gradients in spiders' silk glands, clarifying how spIDroin solubility is maintained and how silk is formed in a fraction of a second.
Liquid crystalline spinning of spider silk
TLDR
Successful copying of the spider's internal processing and precise control over protein folding, combined with knowledge of the gene sequences of its spinning dopes, could permit industrial production of silk-based fibres with unique properties under benign conditions.
Mechanical Properties of Spider Silk.
Threads from spiders may be one of the best structural materials produced by nature. They are used to capture and wrap insects. Less attension has been paid to their mechanical properties of spider…
The mechanical design of spider silks: from fibroin sequence to mechanical function.
TLDR
Comparison of MA silks from Araneus diadematus and Nephila clavipes shows variation in fibroin sequence and properties between spider species provides the opportunity to investigate the design of these remarkable biomaterials.
Structure-function-property-design interplay in biopolymers: spider silk.
...
...