Observation of risk factors, clinical manifestations and genetic characterization of recent Newcastle Disease Virus outbreak in West Malaysia

Abstract

BACKGROUND Newcastle disease virus remains a constant threat in commercial poultry farms despite intensive vaccination programs. Outbreaks attributed to ND can escalate and spread across farms and states contributing to major economic loss in poultry farms. RESULTS Phylogenetic analysis in our study showed that eleven of the samples belonged to genotype VIId. All farms were concurrently positive with two immunosuppressive viruses; Infectious Bursal Disease Virus (IBDV) and Marek's Disease Virus (MDV). Amino acid sequence analysis confirmed that eleven of the samples had sequence motifs for velogenic/mesogenic strains; three were lentogenic. CONCLUSION In conclusion, no new NDV genotype was isolated from the 2011 NDV outbreak. This study suggests that the presence of other immunosuppressive agents such as IBD and MDV could have contributed to the dysfunction of the immune system of the chickens, causing severe NDV outbreaks in 2011. Risk factors related to biosecurity and farm practices appear to have a significant role in the severity of the disease observed in affected farms.

DOI: 10.1186/s12917-015-0537-z

Extracted Key Phrases

9 Figures and Tables

Showing 1-10 of 31 references

Genetic diversity of avian paramyxovirus type 1: Proposal for a unified nomenclature and classification system of Newcastle disease virus genotypes

  • Dg Diel, Lha Silva, H Liu, Z Wang, Pj Miller, Cl Afonso
  • 2012

Molecular characterization of partial fusion gene and C-terminus extension length of haemagglutinin-neuraminidase gene of recently isolated Newcastle disease virus isolates in Malaysia

  • A Berhanu, I Aini, Ar Omar, Mh Bejo
  • 2010

Infectious bursal disease virus strains for vaccination and inactivated bursal tissue origin vaccines. A Lohmann Animal Health News Brief

  • A Bandra
  • 2009