Observation of intersubunit movement of the ribosome in solution using FRET.

  title={Observation of intersubunit movement of the ribosome in solution using FRET.},
  author={Dmitri N. Ermolenko and Zigurts K. Majumdar and Robyn P. Hickerson and Paul Clint Spiegel and Robert M. Clegg and Harry F. Noller},
  journal={Journal of molecular biology},
  volume={370 3},
Protein synthesis is believed to be a dynamic process, involving structural rearrangements of the ribosome. Cryo-EM reconstructions of certain elongation factor G (EF-G)-containing complexes have led to the proposal that translocation of tRNA and mRNA through the ribosome, from the A to P to E sites, is accompanied by a rotational movement between the two ribosomal subunits. Here, we have used Förster resonance energy transfer (FRET) to monitor changes in the relative orientation of the… CONTINUE READING
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