Observation of Changes in Bacterial Cell Morphology Using Tapping Mode Atomic Force Microscopy

@inproceedings{CamesanoObservationOC,
  title={Observation of Changes in Bacterial Cell Morphology Using Tapping Mode Atomic Force Microscopy},
  author={Terri Anne Camesano and Michael J. Natan and Bruce E Logan}
}
Atomic force microscopy (AFM) was used in tapping mode to probe morphological changes in surfaceconfined cells resulting from adhesion-modifying chemicals. Bacteria (Burkholderia cepacia G4 and Pseudomonas stutzeri KC) were exposed to Tween 20, heparin, disodium tetraborate, sodium pyrophosphate, low ionic strength water, lysozyme/ethylenediaminetetraacetic acid (EDTA), and 3-(4-morpholino)propanesulfonic acid sodium salt (MOPS) buffer (as a control), and the surface topography of the cells was… CONTINUE READING

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