Oblique banding pattern in collagen fibrils reconstituted in vitro after trypsin treatment.

@article{Ghosh1975ObliqueBP,
  title={Oblique banding pattern in collagen fibrils reconstituted in vitro after trypsin treatment.},
  author={Sahl K Ghosh and H P Mitra},
  journal={Biochimica et biophysica acta},
  year={1975},
  volume={405 2},
  pages={340-6}
}
Collagen fibres from rat tail tendon suspended in small pieces in a solution (pH 7.8) containing 0.5 M CaCl2 were treated with purified bovine trypsin at 20 degrees C for 20 h. After the enzyme treatment collagen from this solution was precipitated out and reconstituted in vitro into native-type fibrils. The banding pattern in these reconstituted fibrils was found to be oblique. This is comparable to that observed recently in fibrils reconstituted from cartilage collagen. On the other hand… CONTINUE READING