OSP: a computer program for choosing PCR and DNA sequencing primers.

@article{Hillier1991OSPAC,
  title={OSP: a computer program for choosing PCR and DNA sequencing primers.},
  author={LaDeana W. Hillier and Phil Green},
  journal={PCR methods and applications},
  year={1991},
  volume={1 2},
  pages={124-8}
}
OSP (Oligonucleotide Selection Program) selects oligonucleotide primers for DNA sequencing and the polymerase chain reaction (PCR). The user can specify (or use default) constraints for primer and amplified product lengths, %(G+C), (absolute or relative) melting temperatures, and primer 3' nucleotides. To help minimize nonspecific priming and primer secondary structure, OSP screens candidate primer sequences, using user-specifiable cutoffs, against potential base-pairing with a variety of… CONTINUE READING
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