OSBP-Related Proteins (ORPs) in Human Adipose Depots and Cultured Adipocytes: Evidence for Impacts on the Adipocyte Phenotype

@article{Zhou2012OSBPRelatedP,
  title={OSBP-Related Proteins (ORPs) in Human Adipose Depots and Cultured Adipocytes: Evidence for Impacts on the Adipocyte Phenotype},
  author={You Zhou and Marius R. Robciuc and Martin Wabitsch and Anne Juuti and Marja Leivonen and Christian Ehnholm and Hannele Yki-J{\"a}rvinen and Vesa M. Olkkonen},
  journal={PLoS ONE},
  year={2012},
  volume={7}
}
Oxysterol-binding protein (OSBP) homologues, ORPs, are implicated in lipid homeostatic control, vesicle transport, and cell signaling. We analyzed here the quantity of ORP mRNAs in human subcutaneous (s.c.) and visceral adipose depots, as well as in the Simpson-Golabi-Behmel syndrome (SGBS) adipocyte cell model. All of the ORP mRNAs were present in the s.c and visceral adipose tissues, and the two depots shared an almost identical ORP mRNA expression pattern. SGBS adipocytes displayed a similar… 

Figures and Tables from this paper

Oxysterol-binding protein-like 2 contributes to the developmental progression of preadipocytes by binding to β-catenin
TLDR
The data indicated that OSBPL2/ORP2 mediates the transport of β-catenin into the nucleus and thus regulates target genes related to adipocyte differentiation and may be a potential therapeutic target for the treatment of obesity-related diseases.
Free Radical-derived Oxysterols: Novel Adipokines Modulating Adipogenic Differentiation of Adipose Precursor Cells.
TLDR
Free radical-derived oxysterols accumulate in the "diabetic" fat and may act as novel adipokines modulating the adipogenic potential of undifferentiated adipose precursor cells.
Human In Vitro Vascularization, Adipocytes and Vascularized Adipose Tissue : Three relevant models for studies of vascularization, adipocytes and their interaction
TLDR
This differentiation protocol developed provides a promising approach for induction of hASC adipogenesis to obtain functional and mature human adipocytes, which were sensitive to insulin as observed from increased glucose uptake and inhibition of lipolysis.
Development of Versatile Human In Vitro Vascularized Adipose Tissue Model with Serum‐Free Angiogenesis and Natural Adipogenesis Induction
TLDR
A vascularized adipose tissue model is developed where human adipose stromal cells and human umbilical cord vein endothelial cells were cocultured with natural adipogenic and defined serum‐free angiogenic media for 14 days, producing strong lipid accumulation, good vascular network formation and induced adipocyte‐specific protein secretion and expression of studied adipocyte genes.
Blood hsa-miR-122-5p and hsa-miR-885-5p levels associate with fatty liver and related lipoprotein metabolism—The Young Finns Study
TLDR
These miRNAs may be associated with FL formation through the regulation of lipop Protein metabolism as hsa-miR-122-5p levels associated with small VLDL, IDL, and large LDL lipoprotein subclass components, while hsa -885-5P levels associated inversely with XL HDL cholesterol levels.
A guide to studying human dermal adipocytes in situ
TLDR
This practical, user‐friendly guide aims to introduce the techniques available to study human DWAT in situ and ex vivo, including immunohistochemistry, immunofluorescence microscopy and analysis via quantitative Immunohistomorphometry, and offers the reader guidelines on fixing, processing and staining human DAs.
Clinical Value and Potential Mechanisms of Oxysterol-Binding Protein Like 3 (OSBPL3) in Human Tumors
TLDR
It is demonstrated that a novel agent OSBPL3 plays an important role in tumorigenesis from the perspective of publicly available databases and clinical tumor samples in various cancers, which comprehensively provide insights into its biological functions and may be helpful for further investigation.
...
...

References

SHOWING 1-10 OF 66 REFERENCES
OSBP-Related Protein 8 (ORP8) Regulates Plasma and Liver Tissue Lipid Levels and Interacts with the Nucleoporin Nup62
TLDR
The results reveal that ORP8 has the capacity to modulate lipid homeostasis and SREBP activity, probably through an indirect mechanism, and provide clues of an entirely new mode of ORP action.
OSBP-related Protein 8 (ORP8) Suppresses ABCA1 Expression and Cholesterol Efflux from Macrophages*
TLDR
The data identify ORP8 as a negative regulator of ABCA1 expression and macrophage cholesterol efflux and may, thus, modulate the development of atherosclerosis.
Oxysterol Binding Protein Induces Upregulation of SREBP-1c and Enhances Hepatic Lipogenesis
TLDR
It is demonstrated that OSBP regulates hepatic TG metabolism and suggest the involvement of OSBP in the insulin signaling pathways that control hepatic lipogenesis.
Subfamily III of mammalian oxysterol-binding protein (OSBP) homologues: the expression and intracellular localization of ORP3, ORP6, and ORP7
TLDR
Basic characterization of subfamily III, which consists of ORP3, ORP6, and ORP7, shows marked quantitative differences in tissue expression, suggesting tissue-specific aspects in their function and dual targeting of the proteins indicates a putative role in communication between the ER and the plasma membrane.
The proteomic analysis of an adipocyte differentiated from human mesenchymal stem cells using two‐dimensional gel electrophoresis
TLDR
The protein expression inherent to adipogenic differentiation, by 2‐DE, MALDI‐TOF, and RT‐PCR was analyzed and four proteins: syntaxin‐3, OSBP‐related protein 3, PPAR‐γ and glycophorin were associated with adipogenesis.
The two variants of oxysterol binding protein-related protein-1 display different tissue expression patterns, have different intracellular localization, and are functionally distinct.
TLDR
The results suggest that the two forms of ORP1 are functionally distinct and that OrP1L is involved in control of cellular lipid metabolism.
ORP2, a homolog of oxysterol binding protein, regulates cellular cholesterol metabolism.
TLDR
The results implicate ORP2 as a novel regulator of cellular sterol homeostasis and intracellular membrane trafficking.
Characterization of a human preadipocyte cell strain with high capacity for adipose differentiation
TLDR
The human cell strain described here provides an almost unlimited source of human preadipocytes with high capacity for adipose differentiation and may, therefore, represent a unique tool for studying human fat cell development and metabolism.
...
...