ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe

@article{Matsuyama2006ORFeomeCA,
  title={ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe},
  author={Akihisa Matsuyama and R. Arai and Yoko Yashiroda and A. Shirai and Ayako Kamata and S. Sekido and Yumiko Kobayashi and Atsushi Hashimoto and M. Hamamoto and Y. Hiraoka and S. Horinouchi and Minoru Yoshida},
  journal={Nature Biotechnology},
  year={2006},
  volume={24},
  pages={841-847}
}
Cloning of the entire set of an organism's protein-coding open reading frames (ORFs), or 'ORFeome', is a means of connecting the genome to downstream 'omics' applications. Here we report a proteome-scale study of the fission yeast Schizosaccharomyces pombe based on cloning of the ORFeome. Taking advantage of a recombination-based cloning system, we obtained 4,910 ORFs in a form that is readily usable in various analyses. First, we evaluated ORF prediction in the fission yeast genome project by… Expand
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