ORF17 from the clavulanic acid biosynthesis gene cluster catalyzes the ATP-dependent formation of N-glycyl-clavaminic acid.

@article{Arulanantham2006ORF17FT,
  title={ORF17 from the clavulanic acid biosynthesis gene cluster catalyzes the ATP-dependent formation of N-glycyl-clavaminic acid.},
  author={Haren Arulanantham and Nadia J. Kershaw and Kirsty S. Hewitson and Claire E Hughes and Jan E Thirkettle and Christopher J. Schofield},
  journal={The Journal of biological chemistry},
  year={2006},
  volume={281 1},
  pages={279-87}
}
(3R,5R)-Clavulanic acid, a clinically used inhibitor of serine beta-lactamases, is produced by fermentation of Streptomyces clavuligerus. The early steps in clavulanic acid biosynthesis leading to the bicyclic beta-lactam intermediate (3S,5S)-clavaminic acid have been defined. However, the mechanism by which (3S,5S)-clavaminic acid is converted to the penultimate intermediate (3R,5R)-clavaldehyde is unclear. Disruption of orf15 or orf16, of the clavulanic acid biosynthesis gene cluster, blocks… CONTINUE READING

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