OPS vitrification of mouse immature oocytes before or after meiosis: the effect on cumulus cells maintenance and subsequent development.

@article{Suo2009OPSVO,
  title={OPS vitrification of mouse immature oocytes before or after meiosis: the effect on cumulus cells maintenance and subsequent development.},
  author={Lun Suo and Guang-bin Zhou and Qing-gang Meng and Chang-Liang Yan and Zhi-Qiang Fan and X M Zhao and Xiang-wei Fu and Yan-Ping Wang and Qing-jing Zhang and Shi-en Zhu},
  journal={Zygote},
  year={2009},
  volume={17 1},
  pages={71-7}
}
Cryopreservation can cause cumulus cell damage around the immature oocytes, which may result in poor subsequent development. To evaluate the effect of the meiosis stage on the cumulus cell cryoinjury and determine the suitable stage for cryopreservation in immature oocytes, mouse oocytes at germinal vesicle (GV) and germinal vesicle breakdown (GVBD) stages were vitrified using open pulled straw (OPS) method. Cumulus cells damage was scored immediately after thawing by double-fluorescent… CONTINUE READING

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