Corpus ID: 18257053

ON THE ACTION OF COLCHICINE

@article{Malawista1965ONTA,
  title={ON THE ACTION OF COLCHICINE},
  author={S. Malawista},
  journal={The Journal of Experimental Medicine},
  year={1965},
  volume={122},
  pages={361 - 384}
}
  • S. Malawista
  • Published 1965
  • Medicine
  • The Journal of Experimental Medicine
The effect of colchicine was studied on the rapid, reversible darkening of frog skin under the influence of melanocyte-stimulating hormone (MSH). Darkening is due to dispersion of melanin granules in melanocytes and is thought to be accompanied by a gel-to-sol cytoplasmic transformation. After subsequent washing, the skin lightens, with aggregation of melanin granules and cytoplasmic gelation. Preincubation of skin with colchicine had the following effects: 1. Darkening induced by MSH was… Expand
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With histochemical methods an instantaneous and marked inhibitory effect of a single dose of colchicine on the activity of succinic-, lactic-, glucose-6-phosphate-, NADH- and NADPH-dehydrogenase inExpand
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THE EFFECT OF COLCHICINE ON MOSAIC PATTERNS IN CULTURED CELLS
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  • Biology, Medicine
  • The Journal of cell biology
  • 1968
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  • Chemistry, Medicine
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Cytoskeleton, Microtubules, Tubulin and Colchicine: a Review
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References

SHOWING 1-10 OF 33 REFERENCES
SOME EFFECTS OF COLCHICINE UPON THE FIRST CLEAVAGE IN ARBACIA PUNCTULATA
TLDR
Few studies have been made in an effort to analyze the general effects of colchicine on the cell as a whole and particularly from the point of view of the mechanics of cell division. Expand
Cytological effects of colchicine on the grasshopper neuroblast in vitro with special reference to the origin of the spindle
TLDR
It is suggested that colchicine does not destroy the spindle material but merely alters its molecular orientation, so that it comes to comprise a spherical mass with no mitotic function. Expand
THE MECHANISM OF ACTION OF COLCHICINE
TLDR
The properties of the colchicine-bindinG protein, (binding constant, sedimentation constant, Sephadex elution volume) are similar to those obtained with the protein from mammalian cells, sea-urchin sperm tails, and brain tissue, and thus support the conclusion that the protein is a subunit of microtubules. Expand
In vitro bioassay for the melanocyte stimulating hormone.
TLDR
Because mammalian as well as amphibian melanocytes are affected by the pigment-cell activating principle of the pituitary gland, the latter has been designated as melanocyte stimulating hormone (MSH). Expand
STUDIES ON INHIBITION OF THE COLCHICINE EFFECT ON MITOSIS
TLDR
The conclusions reported below on the action of mitosis poisons and their inhibitors are all based on determinations of the mitotic coefficient (ratio of dividing to resting cells), and analyses of the dividing cell population in terms of phase percentages. Expand
On the movement of pigment granules in frog melanocytes.
TLDR
It is suggested that melanin granule movement is produced by cytoplasmic streaming associated with ionic exchange between intra- and extracellular media and that substances affecting melanin dispersion, directly or indirectly produce a change in the specific ionic permeability of the cell membrane. Expand
Suppression of metabolic accompaniments of phagocytosis by colchicine.
TLDR
The beneficial effect of colchicine on gouty inflammation may result from a suppressive effect on leukocyte function, reducing their ability to perform phagocytosis. Expand
The effect of colchicine on striated muscle in tissue culture.
  • G. Godman
  • Biology, Medicine
  • Experimental cell research
  • 1955
Abstract Colchicine, in concentrations of 10−8 M or greater, causes a disruption of sarcoblast ribbons grown in tissue culture. The resulting segments are markedly shortened and widened, and ofExpand
Effect of Colchicine on Resting Cells in Tissue Cultures
  • B. Miszurski, L. Doljanski
  • Biology, Medicine
  • Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
  • 1947
TLDR
Chick embryo heart fibroblasts cultured in vitro, when immersed in colchicine solutions 10-4 or 10-5 M, show a tendency to assume a spherical shape, which is not lethal to the cells and is, to a great extent, reversible. Expand
Desacetylmethylcolchicine in Treatment of Myeloid Leukaemia
Appelbaum, E. (1945). Clinics, 4, 396. and Nelson, J. (1945). J. Amer. med. Ass., 128, 778. and Albin, M. B. (1949). Amer. J. med. Scd.. 218, 260. Bunn, P. A., and Peabody, G. (1952). Arch. intern.Expand
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