O-phenylenediamine oxidation by phorbol myristate acetate-stimulated human polymorphonuclear leukocytes: characterization of two distinct oxidative mechanisms.

@article{Hassan1987OphenylenediamineOB,
  title={O-phenylenediamine oxidation by phorbol myristate acetate-stimulated human polymorphonuclear leukocytes: characterization of two distinct oxidative mechanisms.},
  author={N F Hassan and Donald E. Campbell and Steven D. Douglas},
  journal={Clinical immunology and immunopathology},
  year={1987},
  volume={42 3},
  pages={274-80}
}
O-Phenylenediamine (OPD) oxidation has been extensively utilized for the measurement of peroxidase-mediated catabolism of hydrogen peroxide. However, until now this system has not been evaluated for the measurement of hydrogen peroxide produced upon activation of the hexose monophosphate shunt (HMPS) in polymorphonuclear leukocytes (PMNs). OPD oxidation by phorbol myristate acetate (PMA)-stimulated PMNs was mediated by both hydrogen peroxide and superoxide produced by the activation of the HMPS… CONTINUE READING

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OPD oxidation by phorbol myristate acetate ( PMA)-stimulated PMNs was mediated by both hydrogen peroxide and superoxide produced by the activation of the HMPS .
OPD oxidation by phorbol myristate acetate ( PMA)-stimulated PMNs was mediated by both hydrogen peroxide and superoxide produced by the activation of the HMPS .
PeroxidaseGene product plays role in biological processoxidation
PMA treatment of purified human myeloperoxidase ( MPO ) produced OPD oxidation which is inhibited by superoxide dismutase or 1 mM KCN .
OPD oxidation by phorbol myristate acetate ( PMA)-stimulated PMNs was mediated by both hydrogen peroxide and superoxide produced by the activation of the HMPS .
OPD oxidation by phorbol myristate acetate ( PMA)-stimulated PMNs was mediated by both hydrogen peroxide and superoxide produced by the activation of the HMPS .
OPD oxidation by phorbol myristate acetate ( PMA)-stimulated PMNs was mediated by both hydrogen peroxide and superoxide produced by the activation of the HMPS .
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