O-linked sugar chain of human granulocyte colony-stimulating factor protects it against polymerization and denaturation allowing it to retain its biological activity.

@article{Oheda1990OlinkedSC,
  title={O-linked sugar chain of human granulocyte colony-stimulating factor protects it against polymerization and denaturation allowing it to retain its biological activity.},
  author={Masayoshi Oh-eda and Makoto Hasegawa and Kimihiko Hattori and Hitoshi Kuboniwa and Takashi Kojima and Takuya Orita and K Tomonou and Tsuneo Yamazaki and Norimichi Ochi},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 20},
  pages={
          11432-5
        }
}
Human granulocyte colony-stimulating factor (hG-CSF) is a glycoprotein carrying one O-linked sugar chain. To clarify the role of the oligosaccharide in hG-CSF, some biological and physicochemical properties of the deglycosylated hG-CSF and the intact factor were compared. Recombinant hG-CSF produced in transfected Chinese hamster ovary cells was sequentially digested with neuraminidase and endo-alpha-N-acetylgalactosaminidase. The deglycosylated hG-CSF was one-third as active as the intact form… CONTINUE READING

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