O Serogroup-Specific Touchdown-Multiplex Polymerase Chain Reaction for Detection and Identification of Vibrio cholerae O1, O139, and Non-O1/Non-O139

@article{Bhumiratana2014OST,
  title={O Serogroup-Specific Touchdown-Multiplex Polymerase Chain Reaction for Detection and Identification of Vibrio cholerae O1, O139, and Non-O1/Non-O139},
  author={Adisak Bhumiratana and Achiraya Siriphap and Nutsarin Khamsuwan and Jednipit Borthong and Kaknokrat Chonsin and Orasa Sutheinkul},
  journal={Biochemistry Research International},
  year={2014},
  volume={2014}
}
A novel, sensitive locus-specific touchdown-multiplex polymerase chain reaction (TMPCR), which is based on two-stage amplification pertaining to multiplex PCR and conditional touchdown strategy, was used in detecting and differentiating Vibrio cholerae serogroups. A panel of molecular marker-based TMPCR method generates reproducible profiles of V. cholerae-specific (588 bp) amplicons derived from ompW gene encoding the outer membrane protein and serogroup-specific amplicons, 364 bp for the O1… 
View PDF
7 Citations
Background Citations
2

Figures and Tables from this paper

7 Citations

Citation Type

Citation Type

Direct Detection of Vibrio vulnificus, Vibrio parahaemolyticus, and Vibrio alginolyticus from Clinical and Environmental Samples by a Multiplex Touchdown Polymerase Chain Reaction Assay.
TLDR
This MT-PCR method, which can detect V. vulnificus, V. parahaemolyticus, and V. alginolyticUS directly and simultaneously, was rapid, sensitive, specific, and can be used in clinical diagnostics, food industry studies, and risk assessment of environment.
Characterization and Genetic Variation of Vibrio cholerae Isolated from Clinical and Environmental Sources in Thailand
TLDR
V. cholerae O1 El Tor variant and O139 were pre-dominating the pathogenic strains in Thailand, and Multidrug-resistant strains were observed and conferred resistance to ampicillin, azithromycin, nalidixic acid, sulfamethoxazole, tetracycline, and trimethoprim and harboured variants of the SXT elements.
ISOLATION AND GENOTYPING OF Vibrio cholerae ISOLATES FROM PATIENTS WITH CHOLERA DISEASE IN BABYLON PROVINCE
TLDR
The results revealed that all suspected stool samples showed positive isolation to Vibrio cholera species and Inaba serotype had prevailed in all V. cholerae isolates, while only one Ogawa serotype was reported as a causative agent for cholERA disease in Babylon province.
Genomic analysis of pathogenic isolates of Vibrio cholerae from eastern Democratic Republic of the Congo (2014-2017)
TLDR
Current data confirm the association of both DRC O1 7PET (T)10 sub-clades ST69 and ST515 with recurrent outbreaks in eastern DRC and at regional level over the past 10 years.
Genomic analysis of pathogenic isolates of Vibrio cholerae from eastern Democratic Republic of the Congo (2014-2017)
TLDR
Interestingly, while ST69 is predominantly a locally endemic sequence type, ST515 became adaptable enough to expand across DRC neighboring countries, and is spreading extensively through DRC cross-border countries such as South Sudan, Tanzania, Uganda and Zambia.
Application of a paper based device containing a new culture medium to detect Vibrio cholerae in water samples collected in Haiti.
Desarrollo de un ensayo de PCR para detectar los genes codificadores de la toxina del cólera (ctxAB) en preparaciones del candidato vacunal vivo atenuado CV638

References

SHOWING 1-10 OF 67 REFERENCES
Development and evaluation of a multiplex PCR assay for rapid detection of toxigenic Vibrio cholerae O 1 and O 139
Development and evaluation of a multiplex PCR assay for rapid detection of toxigenic Vibrio cholerae O1 and O139.
TLDR
All the 38 stool samples positive for either O1 or O139 serogroup by PCR assay were also positive for the ctxA amplicon indicating that the O1 and O139 V. cholerae strains have the genetic potential of producing cholera toxin.
Method of DNA extraction and application of multiplex polymerase chain reaction to detect toxigenic Vibrio cholerae O1 and O139 from aquatic ecosystems.
TLDR
A method for DNA extraction from coastal water and a multiplex polymerase chain reaction (PCR) for O1 and O139 serogroup and tcpA/ctxA genes by multiplex PCR offers an efficient system, permitting risk analysis for cholera in coastal areas.
Rapid and specific identification of 5 human pathogenic Vibrio species by multiplex polymerase chain reaction targeted to dnaJ gene.
Touchdown-touchup nested PCR for low-copy gene detection of benzimidazole-susceptible Wuchereria bancrofti with a Wolbachia endosymbiont imported by migrant carriers.
The genes responsible for O-antigen synthesis of vibrio cholerae O139 are closely related to those of vibrio cholerae O22.
A molecular survey on virulence associated genotypes of non-O1 non-O139 Vibrio cholerae in aquatic environment of Tehran, Iran.
Molecular Analysis of Non-O1, Non-O139 Vibrio choleraeAssociated with an Unusual Upsurge in the Incidence of Cholera-Like Disease in Calcutta, India
TLDR
Overall, there was an excellent correlation between the results of ribotyping, RFLP analysis of various genes, andPFGE, with strains belonging to a particular serogroup showing nearly identical restriction patterns and PFGE profiles.
Molecular Evidence of Cholera Outbreak Caused by a Toxigenic Vibrio cholerae O1 El Tor Variant Strain in Kelantan, Malaysia
TLDR
The first molecular evidence that the toxigenic V. cholerae O1 El Tor variant has invaded Malaysia is presented, highlighting the need for continuous monitoring to facilitate early interventions against any potential epidemic by this biotype.
Presence of CTX gene cluster in environmental non-O1/O139 Vibrio cholerae and its potential clinical significance.
TLDR
The emergence of a single environmental isolate in this study containing the pathogenicity genes amongst the diverse non-pathogenic environmental isolates needs to be further studied in the context of V. cholerae pathogenicicity sero-coversion.
...
1
2
3
4
5
...