O-(carboxymethyl)oxime steroidal haptens linked in the C3 position: Study of the characteristics of antibodies against 17α-hydroxyprogesterone and testosterone and of their evolution with time

@article{Forest1976OcarboxymethyloximeSH,
  title={O-(carboxymethyl)oxime steroidal haptens linked in the C3 position: Study of the characteristics of antibodies against 17α-hydroxyprogesterone and testosterone and of their evolution with time},
  author={Haguelone G. Forest and Elisabeth Mappus and Claude-Yves Cuilleron},
  journal={Steroids},
  year={1976},
  volume={28},
  pages={815-827}
}
The production of highly sensitive and specific antisera against 17alpha-hydroxyprogesterone (17 OHP) is reported. 17 OHP was rendered antigenic by covalent linkage to bovine serum albumin through position 3 of the steroid. An unambiguous method to prepare the mono-3-0-(carboxy-methyl) oxime derivative (CMO) of 17 OHP is described starting from 17 OHP-acetate. Antisera raised in rabbits were of high affinity for 17 OHP (Ka = 1 to 2 x 10(10) L/mole) and showed very little (less than 0.7%) or no… CONTINUE READING

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