Nutrient control of insulin secretion in isolated normal human islets.

  title={Nutrient control of insulin secretion in isolated normal human islets.},
  author={Jean-Claude Henquin and Denis Dufrane and Myriam Nenquin},
  volume={55 12},
Pancreatic islets were isolated from 16 nondiabetic organ donors and, after culture for approximately 2 days in 5 mmol/l glucose, were perifused to characterize nutrient-induced insulin secretion in human islets. Stepwise increases from 0 to 30 mmol/l glucose (eight 30-min steps) evoked concentration-dependent insulin secretion with a threshold at 3-4 mmol/l glucose, K(m) at 6.5 mmol/l glucose, and V(max) at 15 mmol/l glucose. An increase from 1 to 15 mmol/l glucose induced biphasic insulin… CONTINUE READING


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