Number of receptor sites from scatchard and klotz graphs: a constructive critique.

@article{Klotz1983NumberOR,
  title={Number of receptor sites from scatchard and klotz graphs: a constructive critique.},
  author={Irving M. Klotz},
  journal={Science},
  year={1983},
  volume={220 4600},
  pages={
          981
        }
}
model" on the basis of objective criteria. * It permits simultaneous analysis of data from multiple curves or multiple experiments. * It can provide tests of similarity of shape of two binding isotherms, and enable one to estimate the relative scaling factors for two or more binding isotherms of similar shape. In summary, the Scatchard plot is no more and no less subject to abuse than other plots which in fact have the same informational content. Graphical methods serve well to provide… 
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References

SHOWING 1-5 OF 5 REFERENCES
Numbers of receptor sites from Scatchard graphs: facts and fantasies.
TLDR
Data for ligand and receptor binding presented in the format of a Scatchard graph are compared with the same data shown as bound ligand plotted against the logarithm of free ligand to show extrapolations in the Scatchards graph to yield total number of receptor sites are generally not correct.
Determination of ligand binding: partial and full saturation of aspartate transcarbamylase. Applicability of a filter assay to weakly binding ligands.
Number of receptor sites from Scatchard and Klotz graphs: a constructive critique
Analysis of macromolecule--ligand binding by determination of stepwise equilibrium constants.
The Binding of Organic Ions by Proteins1