Null Results in Brief No Apparent Association of GSTP1 A 313 G Polymorphism with Breast Cancer Risk among Postmenopausal Iowa Women 1

@inproceedings{Zhao2001NullRI,
  title={Null Results in Brief No Apparent Association of GSTP1 A 313 G Polymorphism with Breast Cancer Risk among Postmenopausal Iowa Women 1},
  author={Ming‐fang Zhao and Ryan Lewis and Deborah Ruth Gustafson and W. Wen and James R. Cerhan and Wei Zheng},
  year={2001}
}
blood cells was used to determine the genotypes of the GSTP1 gene using the PCR-RFLP method. The primers for the PCR reactions were GSTP1 forward 5-ccagtgactgtgtgttgatc-3 and reverse 5-caaccctggtgcagatgctc-3. The PCR reactions were carried out in a 50-l mixture containing sample DNA, 20 mM Tris-HCl (pH 8.4), 5.0 mM KCl, 1.5 mM MgCl2, 0.2 mM deoxynucleotide triphosphate 1 unit of Taq polymerase, and 0.4 M of each oligonucleotide primer. Amplification, which resulted in a 189-bp fragment, was… CONTINUE READING

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