Nucleus–Cytosol Interactions—A Source of Stoichiometric Error in Flow Cytometric Estimation of Nuclear DNA Content in Plants

@inproceedings{Noirot2000NucleusCytosolIS,
  title={Nucleus–Cytosol Interactions—A Source of Stoichiometric Error in Flow Cytometric Estimation of Nuclear DNA Content in Plants},
  author={Michel Noirot and Philippe Georges Barre and Jacques Louarn and Christophe Duperray and Serge Hamon},
  year={2000}
}
Abstract Discrepancies of 2–20% were noted in nuclear DNA content estimates using mixed and unmixed extracts of several plant species combinations (target/standard). This may be because cytosolic components affect dye accessibility to DNA. Several dilution experiments showed clearly how cytosol concentration modified dye accessibility. Heat treatment suggested that dye accessibility could be related to chromatin sensitivity to decondensation. Some methods (nucleus isolation, dilution) can… CONTINUE READING

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