Nucleotides in precursor tRNAs that are required intact for catalysis by RNase P RNAs.

  title={Nucleotides in precursor tRNAs that are required intact for catalysis by RNase P RNAs.},
  author={David L. Thurlow and D Shilowski and Terry L. Marsh},
  journal={Nucleic acids research},
  volume={19 4},
Precursor tRNAAsp molecules, containing a 26-base 5' leader, were treated with diethylpyrocarbonate, 50% hydrazine or anhydrous hydrazine/3M NaCl and then subjected to processing by RNase P RNAs from Escherichia coli or Bacillus subtilis. Fully processed tRNAs and material not successfully cleaved by the catalytic RNAs were analyzed for their content of chemically altered nucleotides. Several bases were identified as being required intact for optimal activity as substrate as judged by exclusion… CONTINUE READING


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