Nucleotide specificity of human deoxycytidine kinase.

  title={Nucleotide specificity of human deoxycytidine kinase.},
  author={Donna S. Shewach and Kristen K. Reynolds and Laura Hertel},
  journal={Molecular pharmacology},
  volume={42 3},
The ability of deoxycytidine kinase (dCK) to phosphorylate 2'-deoxycytidine (dCyd) and its analogs in the presence of eight nucleoside triphosphates (NTPs), simulating the cellular milieu, was investigated. Using highly purified dCK from MOLT-4 T lymphoblasts, Km and Vmax values were determined for the phosphorylation of dCyd in the presence of cellular concentrations of the eight endogenous NTPs. The results demonstrated that the efficiency of dCyd phosphorylation was greatest in the presence… CONTINUE READING


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