Nucleotide sequencing and characterization of the genes encoding benzene oxidation enzymes of Pseudomonas putida.

Abstract

The nucleotide sequence of the genes from Pseudomonas putida encoding oxidation of benzene to catechol was determined. Five open reading frames were found in the sequence. Four corresponding protein molecules were detected by a DNA-directed in vitro translation system. Escherichia coli cells containing the fragment with the four open reading frames… (More)

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